秦川牛 OLR1基因多态性与肉质性状的关联分析

采用PCR-RFLP的方法对230头秦川肉牛基因组的氧化型低密度脂蛋白受体1(OLR1)基因进行研究,并对其基因型与部分肉质性状进行关联分析。结果发现,OLR1基因存在g.10497C>A突变,该突变位点能够被PstI限制性酶识别。群体中共存在3种基因型,分别命名为AA、AC和CC,基因型频率分别为0.287、0.217和0.496。等位基因A和C的基因频率分别为0.396和0.604。χ2检验表明,该基因座处于Hardy-Weinberg不平衡状态(P<0.05)。关联分析结果表明,CC基因型的眼肌面积显著高于AA基因型(P<0.05);同样,CC基因型与高眼肌深度相关(P<0.05)。而AC基因型与AA和CC基因型之间没有显著差异(P>0.05);在背膘厚和肌间脂肪含量这2个性状上,3种基因型之间差异不显著(P>0.05)。

Association Analysis of OLR1Gene and Meat Quality Traits in Qinchuan Cattle

PCR-RFLP was used to identify the genotype of the OLR1gene in 230 Qinchuan cattle genome.A g.10497C>A mutation was identified at OLR1gene,and three genotypes were detected,named AA,AC and CC.The frequencies of the three genotypes were 0.287,0.217 and 0.496,respectively.The frequencies of allele A and C were 0.396 and 0.604.The genotype frequencies were not as expected in Hardy-Weinberg equilibrium by χ2-test(P<0.05).The result of the correlation analysis indicated that CC and AA genotype had significant differences in loin eye area and Marbling depth(P<0.05),the loin eye area and Marbling depth of individuals with genotype CC were significantly better than those of individuals with genotype AA(P<0.05),but the differences in back fat thickness and Muscle fat were not significant(P>0.05).