基于ISSR标记的韭菜种质资源遗传多样性初探

为深化韭菜种质资源遗传多样性研究,应用ISSR分子标记方法,进行韭菜资源遗传多样性和亲缘关系分析。筛选15条多态性好的ISSR引物,对36份韭菜种质资源建立起DNA指纹库,扩增指纹多态性比率达96.3%,是韭菜种质资源研究的一种有效分子标记;应用Nei-li相似系数法估算出36份材料间的遗传相似系数(genetic similarity)为0.60～0.82,遗传多样性比较丰富。可见,ISSR分子标记是进一步开展韭菜种质资源DNA指纹库构建与分子标记辅助育种技术研究的有效手段。

The Primary Exploring of Genetic Diversity of Leek Varieties Based on ISSR Markers

In order to provide theoretical information for leek breeding,this study was to probe the relationship and genetic diversity of the leek germplasms.We screened 36 leek samples with 15 ISSR primers.The first leek genetic diversity DNA fingerprint database was constructed based on the ISSR results.The ISSR amplification diversity reached 96.3%,which could be an effective molecular marker system to study the leek germplasms.The genetic similarity(GS) among 36 leek samples ranged from 0.60 to 0.82,which could fully showed that they have rich genetic diversity.The result indicated that ISSR analysis was an effective molecular marker system for construction DNA fingerprint database and molecular marker assistant breeding.