博落回生物碱提取工艺初步研究

分别以氯仿、甲醇和工业乙醇为溶剂，对博落回全草中的生物碱进行超声萃取，然后采用3种不同的萃取工艺对各粗提取物中的生物碱进行初步分离。通过对各粗提物和萃取物的提取率与生物碱的比较分析，确定出博落回总碱的最佳提取和分离工艺。其最佳工艺为：工业乙醇超声提取，浸膏溶于正丁醇／水(V／V1：1)的混合溶液，并用正丁醇萃取。然后将正丁醇浸膏溶于氯仿／水(V／V1：1)混合溶液，调pH至10～11，再用氯仿萃取脂溶性总碱。此后，将水相调pH至中性再用正丁醇萃取水溶性总碱，总生物碱提取率可达3．1％。

Preliminary Study on Extraction Technology of Alkaloids in Macleaya cordata

Three methods were used to extract the A lkaloids in Macleaya cordata By extraction rate and thin layer chrom atography (TLC)we selected the bestway The results were as follows: The sample was extracted with ethanol and was dissovlved in n-butylalcohol-water(V/V)1:1].Then it was extracted in n-butylalcohol and was solvated in chloroform-water(V/V)1:1].This solvent was extracted by chloroform under condition of pH 10-11.The part of chloroform is the fat-solventing alkaloids and the part of water continually extracted by n-butyl alcohol under condition of pH 6.5~7.0.This part is thewater-solventing alkaloids.The total alkaloidswere 3.1 percent.