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果胶酶活力的测定方法研究
引用本文:张飞,岳田利,费坚,袁亚宏,高振鹏.果胶酶活力的测定方法研究[J].西北农业学报,2004,13(4):134-137.
作者姓名:张飞  岳田利  费坚  袁亚宏  高振鹏
作者单位:西北农林科技大学食品科学与工程学院,陕西,杨凌,712100
基金项目:国家科技部西部专项(2001BA901A19)基金;国家"十五"科技攻关项目(2001BA501A15)基金;霍英东基金(81065)
摘    要:针对QB1502-92标准测定方法测定高活力果胶酶、固定化果胶酶活力存在的实验误差大,重现性及灵敏度差的缺陷,提出了利用3,5-二硝基水杨酸与醛糖共热产生棕红色的氨基化合物,在一定范围内还原糖的量和含有呈色氨基化合物的反应液颜色深浅成正比的原理,在540nm下测其吸光度,从而计算出果胶酶活力的测定方法,并对测定步骤、影响因素及测定中可能出现的问题进行了试验研究。结果表明,该方法简便、准确、重现性高,适合高活力果胶酶和固定化果胶酶活力的测定。

关 键 词:果胶酶活力  测定方法
文章编号:1004-1389(2004)04-0134-04
收稿时间:2004/5/27 0:00:00
修稿时间:2004年5月27日

Research on Measuring Method of PG Activity
ZHANG Fei,YUE Tian-li,FEI Jian,YUAN Ya-hong and GAO Zhen-peng.Research on Measuring Method of PG Activity[J].Acta Agriculturae Boreali-occidentalis Sinica,2004,13(4):134-137.
Authors:ZHANG Fei  YUE Tian-li  FEI Jian  YUAN Ya-hong and GAO Zhen-peng
Institution:College of Food Science and Engineering, Northwest Sic-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;College of Food Science and Engineering, Northwest Sic-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;College of Food Science and Engineering, Northwest Sic-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;College of Food Science and Engineering, Northwest Sic-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;College of Food Science and Engineering, Northwest Sic-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China
Abstract:A method for determinating the activities of pectinase was proposed in this paper.It can produce the red-brown amino compounds when 3, 5-dinitro salicylic acid and aldehyde were heated together.The amino compounds had maxinum absorbency at 540nm.In certain range, there was a good linear relationship bettween the amount of reducing sugar and the color of the reaction mixture The activitiesofpectinase can be calculated by determinitng the absorbency at 540nm.The steps, the factors and otherproblems thatmay occur in the experinentwere studied here.The result showed that themethodwasmore sinple, accurate and it also had good reproducibility.Itwasmore efficient than QB 1502 and other methods, especially in determinating the activities of immobilized pectinase and high activity pectinase
Keywords:Activities of pectinase  Measuring method
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