连翘和板蓝根的致突变作用

为研究连翘和板蓝根的致突变作用,以蚕豆和小鼠为材料,用不同剂量的连翘和板蓝根水煎液为诱变剂,测定微核率和染色体畸变率。结果表明,与对照组相比,不同剂量的中药水煎液能诱发细胞微核率和染色体畸变率的增加(P<0.05或P<0.01),且随处理剂量的增大而增加。在相同剂量下,板蓝根的染色体畸变率和微核率比连翘高。中药水煎液诱导蚕豆根尖细胞产生游离染色体、染色体断片、染色体粘连、染色体桥等多种类型的染色体畸变。高剂量的中药水煎液具有明显的致突变作用,其中板蓝根的致突变效应比连翘强。

Mutation Effects of Weeping Forsythia and Isatis Root

This paper studied the mutation effects of Weeping Forsythia and Isatis root.The micronucleus rate and the rate of chromosome aberration were determined by water solution of Weeping Forsythia and Isatis root in different dosage with Vicia faba and mice.The results showed that the water boiled juice of Weeping Forsythia and Isatis root in different dosage increased the micronucleus rate and the rate of chromosome aberration comparing with the control group(P<0.05 or P<0.01).Both these rates increased with treatment dosage.The micronucleus rate and the rate of chromosome aberration of Isatis root was higher than that of Weeping Forsythia at the some dosage.the water solution of traditional Chinese medicine induced the root tip cells of Vicia faba to produce various types of chromosome aberrations:free chromosome,chromosome fragments,chromosome adhesion,chromosome bridge.The water solution of traditional Chinese medicine at high dosage have obvious mutation effect.The mutation effect of Isatis root is stronger than Weeping Forsythia.