太湖鸡PRL、PRLR和FSHβ基因多态与前期产蛋性状关系研究

采用PCR-SSCP法对太湖鸡催乳素(PRL)、催乳素受体(PRLR)和促卵泡激素β亚基(FSHβ)基因进行多态性检测,并分析其与开产50%后20周产蛋性状的关系。结果表明:PRL和FSHβpro2的基因型频率都是AA型最高,BB型最低,等位基因频率也是A高于B,FSHβ基因exon3位点没有多态;PRLR只有两种基因型,外显子3是AA型频率高于BB型,外显子6是CC型频率高于DD型,且等位基因A和C的频率分别高于等位基因B和D的频率。太湖鸡PRL、PRLR和FSHβ基因开产50%后20周产蛋性状的不同基因型间差异均不显著(P>0.05),但是PRLR基因外显子3的开产50%后9~11周蛋重平均数基因型间差异接近显著(P=0.08)。基因间交互作用分析发现,催乳素受体基因外显子3和外显子6有极显著的交互作用(P<0.01),产蛋量较高的合并基因型AADD可以作为太湖鸡选育的参考。

Polymorphisms in Taihu Chicken of PRL, PRLR and FSHβ Genes and Association with Prophase Egg Production

The PCR-SSCP method was applied to test the polymorphism of prolactin(PRL),prolactin receptor (PRLR)and follicle-stimulating hormone beta (FSHB)genes in Taihu chicken,and the relationship between the genotypes of PRL, PRLR and FSH Band 20 weeks egg production traits after the age at 50% egg in this population was analyzed.The results showed that genotype AA frequency was higher than that of genotype BB in PRL and FSHB pro-genes, the allele A frequency was higher than B frequency, no polymorphism existed in FSHB exon3; There where two genotypes in PRLR, and genotype AA frequency was higher than that of genotype BB in exon3,genotype CC frequency was higher than that of genotype DD in exon6; the allele A/C frequency was higher than B/D frequency respective 1v.No significant association was found for the PRL, PRLP and FSHBgenes with the eggproduction traits after the age at 50% egg(P>0.05).But the P value of the salience test of the egg weight average at 9211 weeks after the age at 50% egg was 0.08 in PRLR exon3.Significant interactions between PRLR exon3 and PRLRexon6 were detected in gene interaction analysis(P<0.01),and the incorporative genotype AADD is available to breed TaiHu chicken.