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蝙蝠葛茎叶正丁醇提取物抑菌作用研究
引用本文:刘瑞,马养民,张弘弛,陈玲,史清华.蝙蝠葛茎叶正丁醇提取物抑菌作用研究[J].西北农业学报,2007,16(1):77-81.
作者姓名:刘瑞  马养民  张弘弛  陈玲  史清华
作者单位:1. 西北农林科技大学生命科学学院,陕西杨凌,712100
2. 西北农林科技大学生命科学学院,陕西杨凌,712100;陕西科技大学化学与化工学院,陕西咸阳,712081
3. 西北农林科技大学林学院,陕西杨凌,712100
摘    要:采用活性追踪法研究了蝙蝠葛(Menispermum dahuricum DC.)茎叶的正丁醇提取物及其柱层析分离得到的6个组分(F1、F2、F3、F4、F5和F6)对2种细菌、2种酵母菌和9种霉菌的抑制作用。结果表明,一定浓度的正丁醇提取物对供试的细菌、酵母菌和真菌都有较明显的抑制作用。柱层析得到的F1与F2具有显著的抑菌活性。同时,蝙蝠葛茎叶的正丁醇提取物和F1、F2对食品有一定的防腐效果,可以作为天然食品防腐剂开发利用。

关 键 词:蝙蝠葛  提取物  抑菌活性  柱层析  防腐剂
文章编号:1004-1389(2007)01-0077-05
收稿时间:2006-07-14
修稿时间:2006-09-15

Study on the Antimicrobial Activities of the n-butanol Extract from Leaves and Stem of Menispermum dahuricum DC.
LIU Rui,MA Yang-min,ZHANG Hong-chi,CHEN Ling and SHI Qing-hua.Study on the Antimicrobial Activities of the n-butanol Extract from Leaves and Stem of Menispermum dahuricum DC.[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(1):77-81.
Authors:LIU Rui  MA Yang-min  ZHANG Hong-chi  CHEN Ling and SHI Qing-hua
Institution:College of Life Science, Northwest A &F University, Yangling Shaanxi 712100, China;College of Life Science, Northwest A &F University, Yangling Shaanxi 712100, China;College of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xianyang Shaanxi 712081, China;College of Life Science, Northwest A &F University, Yangling Shaanxi 712100, China;College of Life Science, Northwest A &F University, Yangling Shaanxi 712100, China;College of Forestry, Northwest A &F University, Yangling Shaanxi 712100, China
Abstract:The antimicrobial effects of the n-butanol extract from leaves and stem of M.dahuricum DC,and 6 fractions(F1,F2,F3,F4,F5,F6) from the n-butanol extract by column chromatography against two kinds of germs,two kinds of yeasts and nine kinds of molds were accomplished.The results indicated that the extract had antimicrobial effect,and F1 and F2 had strong antimicrobial activities.Meanwhile,the n-butanol extract,F1 and F2 had antisepsis effect for food,and can be developed to be a natural food antiseptic agent.
Keywords:Menispermum dahuricum DC  Extract  Antimicrobial activity  Column chromatography  Antiseptic agent
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