| 砂地柏内生真菌SC13菌株的分离鉴定及代谢产物初步研究 |
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| 引用本文： | 卢亮,何军,余鑫平,李广泽,张兴.砂地柏内生真菌SC13菌株的分离鉴定及代谢产物初步研究[J].西北农业学报,2006,15(5):85-89. |
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| 作者姓名： | 卢亮 何军 余鑫平 李广泽 张兴 |
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| 作者单位： | 1. 西北农林科技大学无公害农药研究服务中心,陕西杨凌,712100
2. 西北农林科技大学无公害农药研究服务中心,陕西杨凌,712100;陕西省生物农药工程技术研究中心,陕西杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 为了寻找可产生鬼臼毒素类化合物的内生真菌,从砂地柏(Sabina vulgarisAnt.)不同组织中分离到126株内生真菌。以鬼臼毒素,脱氧鬼臼毒素和苦鬼臼毒素为标准品,采用HPLC法筛选得到一株可代谢产生重要药用和杀虫活性物质鬼臼毒素类似物的内生真菌SC13。对菌株SC13进行ITS-5.8S rDNA区域扩增,测序及遗传进化关系分析,初步推测该菌株属于链格孢属(Alternariasp.)真菌。采用小叶碟添加法测定该菌株发酵液萃取物对三龄初期粘虫的拒食活性,其48h拒食中浓为7.259 mg.mL-1,采用浸毒叶片法测定了三龄初期小菜蛾的毒杀活性,其48h致死中浓为6.136 mg.mL-1。
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| 关 键 词： | 砂地柏 内生真菌 鬼臼毒素类似物 生物活性 链格孢属 |
| 文章编号： | 1004-1389（2006）05-0085-05 |
| 收稿时间： | 2006-03-31 |
| 修稿时间： | 2006-05-09 |
Studies on Isolation and Identification of Endophytic Fungi Strain SC13 from Harmaceutical Plant Sabina vulgaris Ant. and Metabolites |
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| LU Liang,HE Jun,YU Xin-ping,LI Guang-ze and ZHANG Xin.Studies on Isolation and Identification of Endophytic Fungi Strain SC13 from Harmaceutical Plant Sabina vulgaris Ant. and Metabolites[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(5):85-89. |
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| Authors: | LU Liang HE Jun YU Xin-ping LI Guang-ze and ZHANG Xin |
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| Institution: | Biorational Pesticide R & D Center of Northwest A & F University, Yangling Shaanxi 712100, China;Biorational Pesticide R & D Center of Northwest A & F University, Yangling Shaanxi 712100, China;Shaanxi Research Center of Biopesticide Technology and Engineering, Yangling 712100, China;Biorational Pesticide R & D Center of Northwest A & F University, Yangling Shaanxi 712100, China;Biorational Pesticide R & D Center of Northwest A & F University, Yangling Shaanxi 712100, China;Biorational Pesticide R & D Center of Northwest A & F University, Yangling Shaanxi 712100, China;Shaanxi Research Center of Biopesticide Technology and Engineering, Yangling 712100, China |
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| Abstract: | 126 strain endophytic fungi isolated from healthy organism of pharmaceutical plant Sablrca vulgarls Ant.The culture were extracted with chloroform and analysed by HPLC.The endophtytic fungi stain SC13 couldproducepicropodophyllotoxin.The antifeedant activity against the 3rd larvae of Mythlmrcaseparate Walker.were assayed with leaf-disk method and the poisionous activity against the 3rd larvae of Plutella xylostella L.were assayed with Leaf Dipped Method.The bioassay results showed that the activity of the extracting mixture of strain SC13 had some activities,the AFC50 aganinst the 3rd larvae of Mythlmrca separate Walker.during 48h was 7.23 mg·mL-1,and LC50 against the 3rd larvae of Plutella xylostella during 48 h was 7.23 mg·mL-1.ITS-5.8S rDNA sequence were used to characterize the stain SC13,and showed that the stain SC13 belong to Alterrcaria. |
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| Keywords: | ITS-5 8S rDNA |
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