| 基于真空渗入法的转基因大白菜植株的获得 |
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| 引用本文： | 赵 静,吴俊清,李庆飞,张 静,张鲁刚.基于真空渗入法的转基因大白菜植株的获得[J].西北农业学报,2017(2):248-254. |
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| 作者姓名： | 赵 静 吴俊清 李庆飞 张 静 张鲁刚 |
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| 作者单位： | （1.西北农林科技大学 园艺学院，农业部西北地区园艺作物生物学与种质资源创新重点实验室，陕西杨凌 712100；2.山西农业大学 园艺学院，山西太古 030800） |
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| 基金项目： | 国家科技支撑计划（2012BAD02B015）；西北农林科技大学唐仲英育种基金；陕西省蔬菜产业体系。 |
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| 摘 要： | 采用真空渗入法,将萝卜VPE1基因的干扰载体pRNAi-RsVPE1转入大白菜中,共收获2 414粒种子,在质量浓度为30mg/L的卡那霉素筛选下获得抗性株297株,其中9株经PCR检测呈阳性,转化率为0.37%。Real-time PCR检测结果表明,pRNAi-RsVPE1的转入导致转基因大白菜叶片中同源的BraVPE在RNA水平上的表达量下降,验证了转基因植株的可靠性,为进一步深入研究BraVPE基因功能奠定了基础。
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| 关 键 词： | 真空渗入法 大白菜 BraVPE转基因 RNAi载体 |
Obtaining Transgenic Plants of Chinese Cabbage Based on Vacuum Infiltration |
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| ZHAO Jing,WU Junqing,LI Qingfei,ZHANG Jing and ZHANG Lugang.Obtaining Transgenic Plants of Chinese Cabbage Based on Vacuum Infiltration[J].Acta Agriculturae Boreali-occidentalis Sinica,2017(2):248-254. |
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| Authors: | ZHAO Jing WU Junqing LI Qingfei ZHANG Jing and ZHANG Lugang |
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| Abstract: | The vector pRNAi- RsVPE1 was transformed into Chinese cabbage by vacuum infiltration method and total 2 414 seeds were obtained. The kanamycin concentration 30 mg/L was determined to be the most suitable for selecting transgenic plants. 297 transgenic plants resistant to kanamycin were obtained,of which 9 positive transgenic plants were verified by PCR detection,and the transgenic rate was 0.37%. RT-PCR detection showed that the expression level of BraVPE in transgenic plants decreased significantly,reliability of transgenic plants was verified. Therefore ,the above results laid a foundation for further study in BraVPE gene functions. |
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| Keywords: | Vacuum infiltration Chinese cabbage BraVPE Genetic transformation RNAi vector |
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