| SSR标记辅助回交选育抗草甘膦油菜新品系的研究 |
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| 引用本文： | 张 荣,信晓阳,胡胜武.SSR标记辅助回交选育抗草甘膦油菜新品系的研究[J].西北农业学报,2016,25(1):64-71. |
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| 作者姓名： | 张 荣 信晓阳 胡胜武 |
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| 作者单位： | （1.旱区作物逆境生物学国家重点实验室，陕西杨凌 712100；2.西北农林科技大学 农学院，陕西杨凌 712100） |
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| 基金项目： | 现代农业产业技术体系建设专项资金（CARS 13）；“十一五”国家科技支撑计划（2010BAD01B02）。 |
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| 摘 要： | 为快速培育抗除草剂草甘膦的油菜新品系,以含草甘膦氧化还原酶基因gox的转基因抗草甘膦材料C9为供体亲本,以优良甘蓝型冬油菜Gr-1、Gr-2为轮回亲本,以回交转育为基本方法,利用gox基因的PCR检测和喷除草剂2种方法对回交后代进行前景选择,并利用SSR分子标记进行背景辅助选择,将抗草甘膦基因gox导入Gr-1、Gr-2中,在BC3选育得到遗传背景回复率大于96%的抗草甘膦油菜新品系。
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| 关 键 词： | 油菜 草甘膦抗性 回交转育 SSR标记 辅助选择 |
Development of New Transgenic Glyphosate Resistant Rapeseed Lines Assisted by SSR Marker Selection |
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| ZHANG Rong,XIN Xiaoyang and HU Shengwu.Development of New Transgenic Glyphosate Resistant Rapeseed Lines Assisted by SSR Marker Selection[J].Acta Agriculturae Boreali-occidentalis Sinica,2016,25(1):64-71. |
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| Authors: | ZHANG Rong XIN Xiaoyang and HU Shengwu |
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| Abstract: | By using Canadian rapeseed (Brassica napus L.) line C9 with glyphosate resistance which conferred by the gox gene as the donor parent, and Gr 1,Gr 2, two elite local rapeseed lines as recurrent parents, glyphosate resistance was successfully transferred into Gr 1 and Gr 2 backgrounds by combination of detection of gox gene by PCR (Polymerase Chain Reaction) method and the spraying glyphosate solution method, and SSR (simple sequence repeats) marker assistant background selection. In BC3, glyphosate resistant plants with >96% genetic background recovery rates were successfully developed. |
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