内生枯草芽孢杆菌E1R-J发酵条件的优化

细菌E1R-J是一株对小麦全蚀病、小麦白粉病和小麦条锈病有拮抗作用的小麦内生芽孢杆菌,笔者对其产菌的发酵培养条件进行了初步研究.发酵培养基为酵母粉0.6%,蛋白胨0.6%,NaCl 0.3%,K2HPO40.02%.最佳发酵条件为pH 4.5～9.0,菌株都可以生长,转速150 r/min,温度34℃,种子液的接种量为2%,通气量是空气与培养基的比例2:5,最佳种子液菌龄是20～34 h,作为生防用菌最佳菌龄为48～73 h.在以上条件下,细菌的发酵产量最大,细菌浓度为5.2×107cfu/mL左右.

Optimizing of Fermentation Conditions of Endophytic Bacteria E1R-J

The endophytic bacteria E1R-J is isolated from the root of winter wheat,which has the better effect to take all,powdery mildew and rust of wheat.And that the optimizing of the fermentation conditions showed that the optimum media is that made of pept one 0.6%,yeast exact 0.6%,NaCl 0.3%,K₂HPO₄ 0.02% with initial pH 4.5~9.0,culturing at 34,150r/min,and inoculation of seeds culture with 2%,in 100 mL LB of 250 mL flask,the time of seeds culture is 20~34 h,and the time of fer mentation for biocontrol is 48~73 h.So the concentration is 5.2×10⁷ cfu/mL.