一株韭菜内生高效氯氰菊酯降解生防细菌的分离与鉴定

从新鲜韭菜的根中分离到一株对多种蔬菜病原真菌有抑制作用的优势内生细菌W7,对其拮抗机制的初步研究结果表明,胞外代谢物对病原真菌无抑制作用,而菌体经超声波破碎及有机溶剂沉淀得到的多糖粗提液可明显抑制病原菌菌丝生长。该菌还能以100 mg/L高效氯氰菊酯为唯一碳源生长,7 d的降解率为51.3%,表明该菌是一株兼具农药降解特性的生防内生细菌。通过对其形态特征、生理生化及16S rDNA同源性序列分析,初步鉴定该菌为类芽孢杆菌(Paenibacillus spp.)。

Isolation and Identification of a Beta cypermethrin degrading and Biocontrol Endophytic Bacteria in Leek

One dominant endophytic bacterial strain W7 was isolated from the root of fresh leek,which had a broad inhibition spectrum against several vegetable pathogenic fungi.The results of its antagonistic mechanism showed that the extracellular metabolites had no effect on pathogenic fungi,but the somatic polysaccharose which obtained from cells by ultrasonic disruption and organic solvent precipitation could inhibit the growth of mycelium.Strain W7 was also found to be capable of utilizing beta-cypermethrin as th...