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发根农杆菌诱导黄芩毛状根的研究
引用本文:齐香君,陈秀清,何恩铭.发根农杆菌诱导黄芩毛状根的研究[J].西北农业学报,2006,15(5):244-247.
作者姓名:齐香君  陈秀清  何恩铭
作者单位:陕西科技大学生命科学与工程学院,陕西咸阳,712081
摘    要:利用发根农杆菌A4,A4 9615,1.2556诱导中药黄芩外植体,得到黄芩不定根,根据形态和在MS培养基中进行培养证实得到黄芩毛状根,经抗菌素氨苄西林钠500 mg/L除菌后,毛状根表现出分枝多,生长快,且能在MS中生长繁殖的特性,为采用生物技术生产黄芩活性成分奠定基础。

关 键 词:黄芩  发根农杆菌  毛状根  诱导
文章编号:1004-1389(2006)05-0244-04
收稿时间:2006-03-09
修稿时间:2006-03-30

Research of Hairy Roots Induced Scutellaria baicalensis Georgi by Agrobacterium rhizogenes
QI Xiang-jun,CHEN Xiu-qing and HE En-ming.Research of Hairy Roots Induced Scutellaria baicalensis Georgi by Agrobacterium rhizogenes[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(5):244-247.
Authors:QI Xiang-jun  CHEN Xiu-qing and HE En-ming
Institution:College of Life Science and Engineering, Shaanxi University of Science and Technology, Xianyang 712081,China
Abstract:Hairy roots were induced from Scutellaria baicalensis Georgi by three kinds of Agrobacterium rhizogenes strains A4,A4 9615 and 1.2556.They were revealed by appearance and growing on hormone-free medium.There are some significant characteristics of hairy roots after eliminating bacteria with antibiotic,such as more branches,fast growth capacity and growth in hormone-free media.It is the basis for producing active components of Scutellaria baicalensis Georgi by biotechnology.
Keywords:Scutellaria baicalensis Georgi  Agrobacterium rhizogenes  Hairy roots  Induct
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