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斑点鲶鱼的基因图谱及遗传标记选育研究
引用本文:刘占江,冯纪年.斑点鲶鱼的基因图谱及遗传标记选育研究[J].西北农业学报,2001,10(2):110-114.
作者姓名:刘占江  冯纪年
作者单位:1. 美国奥本大学鱼类分子遗传及生物工程研究室
2. 西北农林科技大学昆虫研究所
摘    要:本文主要总结斑点鲶鱼基因组学研究的最新动态。斑点鲶鱼和蓝色鲶鱼是杂交可育的两个近缘种 ,这一杂交体系不仅为数量性状的表型带来最大的变异 ,同时也为基因型的多样性提供了最大的可能性。利用 AF L P、RAPD、微卫星顺序标记、EST以及 DNA单体多样性标记 ,以建立遗传连锁图。旨在克隆抗病基因、生长基因、抗逆基因、以及控制食物转化率、产肉率、收获率数量性状的基因。另外还对于适合于鲶鱼基因组研究的 DN A指纹技术进行了评估。并通过极端表型、基因型的分析鉴定出了与生长速度、食物转化率及抗病性有连锁的遗传标记。使用基因型分析、辐射细胞溶合及 BA C基因库 ,以建立斑点鲶鱼的遗传连锁图及物理图 ,并用基因薄膜技术、基因芯片技术 ,以进一步掌握在特定生理条件下的基因调控

关 键 词:斑点鲶鱼  基因组学  基因连接  基因图谱  数量遗传定位
文章编号:1004-1389(2001)02-110-05
收稿时间:1999/10/19 0:00:00
修稿时间:1999年10月19

Gene Mapping and Marker-assisted Selection in Channel Catfish(Ictalurus Punctatus)
LIU Zhan-jiang and FENG Jin-nian.Gene Mapping and Marker-assisted Selection in Channel Catfish(Ictalurus Punctatus)[J].Acta Agriculturae Boreali-occidentalis Sinica,2001,10(2):110-114.
Authors:LIU Zhan-jiang and FENG Jin-nian
Institution:LIU Zhan-jiang 1,FENG Jin-nian 2
Abstract:Channel catfish and blue catfish are closely related species,from which fertile progenies can be made.The interspecific hybrid system,therefore,offers and ideal system for genetic linkage analysis because the system not only offers maximum contrast for phenotypic evaluation,but also provide maximum likelihood for genetic marker polymorphism.We have used several DNA fingerprinting technologies including microsates,amplified fragment length polymorphism(AFLP),random amplified polymorphic DNA (RAPD),EST polymorphism(SUuP)to construct a genetic linkage map of catfish.The long term objectives of the research are to understand genomic structure,organization,composition,and evolution,to identify markers that are linked to phenotypic traits for application in marker-assisted selection,to identifygenes involved in various physiological processes and understand regulation of gene expression,and to isolate and clone important genesinvolved in disease resistance,growth,feed conversion efficiency,body conformation and processing yields,tolerance to low dissolved oxygen,tolerance to low water quality,harvestability,and other econmic traits. We have evaluated several types of polymorphic markers and devloped over 70 allozyme markers,600 PAPD markers,3000 AFLP markers,and 250 microsatellite markers.Application of such polymorphic markers using selective genotyping allowed identification of markers that are linked to disease resistance,growth rate,and feed conversion efficiency.These markers should be useful for marker-assisted selection in breeding programs.Cenetic linkage mapping using reference families and redia tion hybrid panels are underway.Large-scale characterization of EST s are being carried out and genes involved in resistance reactions are being identified by using gene chips and gene filters.Details for marker development,phenotype-genotype linkage,identification of QTL-linked markers,and progress in functional genomics will be presented.
Keywords:Ictalurus punctatus  Genomics  Genetic linkage  Quantitative trait loci  Mapping  
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