| 提高棉花花粉管通道法转化率的研究 |
| |
| 引用本文： | 马盾,黄乐平,黄全生,孟庆玉,危晓薇,陈勋基,美丽古丽.提高棉花花粉管通道法转化率的研究[J].西北农业学报,2005,14(1):10-12. |
| |
| 作者姓名： | 马盾 黄乐平 黄全生 孟庆玉 危晓薇 陈勋基 美丽古丽 |
| |
| 作者单位： | 新疆农科院核技术生物技术研究所,乌鲁木齐,830091 |
| |
| 基金项目： | 新疆维吾尔自治区自然科学基金资助项目 |
| |
| 摘 要： | 针对棉花不同受体材料、果台、棉铃大小、注射时间以及操作技术的研究 ,逐步总结出一套适合于新疆的实用操作方法。使外源 DNA通过花粉管通道导入技术得到了不断提高 ,注射棉铃未落铃率平均达到2 5 %以上 ,最高达到 5 6% ,大大提高了外源 DNA的转化率 ,获得了针对性强的高表达率转化棉花植株
|
| 关 键 词： | 棉花 花粉管通道法 转化率 |
| 文章编号： | 1004-1389(2005)01-0010-03 |
| 收稿时间： | 2004/1/6 0:00:00 |
| 修稿时间： | 2004年1月6日 |
Study on Improvement of Pollen Tube Pathway Transformation Efficiency Through Field Concrete Operation |
| |
| MA Dun,HUANG Le-ping,HUANG Quan-sheng,MENG Qing-yu,WEI Xiao-wei,CHEN Xun-ji and Meiliguli.Study on Improvement of Pollen Tube Pathway Transformation Efficiency Through Field Concrete Operation[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(1):10-12. |
| |
| Authors: | MA Dun HUANG Le-ping HUANG Quan-sheng MENG Qing-yu WEI Xiao-wei CHEN Xun-ji and Meiliguli |
| |
| Institution: | Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China;Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China;Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China;Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China;Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China;Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China;Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China |
| |
| Abstract: | Cotton is big embryo sac plant with multiembry, using pollen tube pathway transfer exogenous DNA, one flower can get many seeds. After many years systematic studying and grouping in field, taking different breed variety and different fruit spur and advanced transfer technique, a useful and economic transfer method was got. The transformation efficiency of exogenous DNA was gradually improved. The transferred boll`s rate of existing can up to 25 percent, some can up to 56 percent. Many high expression plants were acquired. |
| |
| Keywords: | Cotton Pollen tube pathway Rate of transfer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
