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| 畜产品中沙门氏菌的快速检测 | |
| 引用本文： | 刘华伟,马立农,郭蔼光,邱立,张春柳,梁顺兴.畜产品中沙门氏菌的快速检测[J].西北农业学报,2004,13(4):32-36. |
| 作者姓名： | 刘华伟 马立农 郭蔼光 邱立 张春柳 梁顺兴 |
| 作者单位： | 1. 深圳职业技术学院,广东,深圳,518055;西北农林科技大学,陕西,杨凌,712100 2. 深圳职业技术学院,广东,深圳,518055 3. 西北农林科技大学,陕西,杨凌,712100 |
| 摘 要： | 以热裂解法快速提取DNA模板,应用PCR技术检测沙门氏菌,22种沙门氏菌的基因均获得特异性扩增(496bp),5种非沙门氏菌检测结果为阴性。试验结果表明,PCR是一种快速、敏感和高度特异性的沙门氏菌检测方法,值得在畜产品沙门氏菌的检测中推广应用。 |
| 关 键 词： | 畜产品 沙门氏菌 热裂解法 PCR 快速检测 |
| 文章编号： | 1004-1389(2004)04-0032-05 |
| 收稿时间： | 2004/9/6 0:00:00 |
| 修稿时间： | 2004年9月6日 |
Rapid Detection of Salmonella by PCR in Domestic Animal Products |
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| LIU Hua-wei,MA Li-nong,GUO Ai-guang,QIU Li,ZHANG Chun-liu and LIANG Shun-xing.Rapid Detection of Salmonella by PCR in Domestic Animal Products[J].Acta Agriculturae Boreali-occidentalis Sinica,2004,13(4):32-36. | |
| Authors: | LIU Hua-wei MA Li-nong GUO Ai-guang QIU Li ZHANG Chun-liu and LIANG Shun-xing |
| Institution: | LIU Hua-wei~ |
| Abstract: | In this experiment DNA templates were extracted rapidly by the hot dehiscing, all 22 salmonella strains were accurately identified 496 bp segment and all 5 non-Salmonella were negative by the PCR. The result showed that PCR is a kind of rapid, sensitive and high specific method to detect salmonella samples, which can be well popularized in detection for salmonella in domestic animal product. |
| Keywords: | Domestic animal product Salmonella Hot dehiscing PCR Rapid detection |
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