布氏杆菌单克隆抗独特型抗体细胞株建立及免疫原性测定

在建立了布氏杆菌单克隆抗体细胞株的基础上,筛选具有强保护作用的单抗细胞株A7免疫BALB/C鼠,并进行细胞融合.用竞争抑制ELISA法筛选出5个强阳性孔,经克隆化培养后首次建立了9个布氏杆菌单克隆抗独特型抗体细胞株.其中,F9株传25代后及液氮冻存复苏后仍能稳定地产生高效价的抗独特型抗体.其IgG类型鉴定为IgG2a,并证实具有抗原“内影象”.以鼠红细胞为载体将抗独特型抗体吸附其表面进行小鼠免疫试验,检测结果表明该抗独特型抗体具有良好的免疫原性,能诱导免疫小鼠产生相当效价抗布氏杆菌抗体(Ab3).

THE ESTABLISHMENT AND IMMUNOGENICITY TEST OF MONOCLONE ANTI-IDIOTYPIC ANTIBODIES TO BRUCELLA

After the establishment of Mcabs to a local brucella strain S₈₅A,the McAb A7 was selected and tested as a protective one.Using McAb A7 to immunize BALB/c mice in order that cell fusion was carried out.Five positive wells were selected out by competitive inhibiting ELISA.After cloning culture we established 9 monoclone anti-idiotypic cell strains.F9, one of them, could prodace higher titer anti-idiotypic Ab.than others did and could do so stably after 25 generations cuture and frozen-thaw procedure.And this anti-idiotypic Ab.was proved to possess "internal image" of S₃₅A while its IgG subtype was identified as IgG2a.The test of immunizing BALB/c mice was conducted by using the red cell of mice as a carrier to conjugate the anti-idiotypic Ab.to it.Those mice had produced certain titer Ab to S₃₅A (A63).This result suggests that the antiidiotypic Ab.F9 not only possess the Ag "internal image",but have the available immunogenicity to stimulate mice producing Ab3.