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| 小麦秆锈病细胞间隙液的提取及注射技术研究 | |
| 引用本文： | 徐建强,侯颖,曹远银,林晓民,周雷.小麦秆锈病细胞间隙液的提取及注射技术研究[J].西北农业学报,2006,15(2):66-68. |
| 作者姓名： | 徐建强 侯颖 曹远银 林晓民 周雷 |
| 作者单位： | 1. 河南科技大学林学院,洛阳,471003 2. 河南科技大学食品与生物工程学院,洛阳,471003 3. 沈阳农业大学植物免疫研究所,沈阳,110161 |
| 基金项目： | 科技部专项基金;河南科技大学校科研和教改项目 |
| 摘 要： | 通过真空渗入离心法提取小麦秆锈病病组织的细胞间隙液,并采用注入法将细胞间隙液注入到健康的小麦细胞,研究了小麦秆锈病细胞间隙液对小麦叶片细胞HR的诱导作用,明确了细胞间隙液的提取和注射技术在小麦秆锈病细胞间隙液研究中利用的可行性。 |
| 关 键 词： | 小麦秆锈病 细胞间隙液 寄主病原互作 |
| 文章编号： | 1004-1389（2006）02-0066-03 |
| 收稿时间： | 2005-10-25 |
| 修稿时间： | 2005-12-10 |
Studies on the Extracting and Injecting Technique of IWF for Puccinia graminis f. sp. tritici |
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| XU Jian-qiang,HOU Ying,CAO Yuan-Yin,LIN Xiao-min and ZHOU Lei.Studies on the Extracting and Injecting Technique of IWF for Puccinia graminis f. sp. tritici[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(2):66-68. | |
| Authors: | XU Jian-qiang HOU Ying CAO Yuan-Yin LIN Xiao-min and ZHOU Lei |
| Institution: | Collge of Forestry, He''nan University of Science and Technology, Luoyang 471003 China;College of Food and Engineering, He''nan Univerity of Science and Technology, Luoyang 471003 China;Institute of Plant Immuniy, Shenyang University of Agriculture, Shenyang 110161 China;Collge of Forestry, He''nan University of Science and Technology, Luoyang 471003 China;Institute of Plant Immuniy, Shenyang University of Agriculture, Shenyang 110161 China |
| Abstract: | Primary leaves of wheat infected by the Puccinia graminis f.sp.tritici were detached,infiltrated with redistilled water,and centrifuged to yield intercellular washing fluids(IWF).IWF were then injected into the healthy wheat leaves.The result showed the induction of IWF to the hypersensitive reaction(HR) on wheat leaves.Two methods were available in the research of IWF. |
| Keywords: | Wheat stem rust Intercellular washing fluids The interaction between wheat and stem rust |
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