|
|||||
|
|
| 卡那霉素在转基因小麦后代筛选中的应用研究 | |
| 引用本文： | 奚亚军,候文胜,张启发,路明.卡那霉素在转基因小麦后代筛选中的应用研究[J].西北农业学报,2002,11(3):17-20. |
| 作者姓名： | 奚亚军 候文胜 张启发 路明 |
| 作者单位： | 1. 西北农林科技大学农学院,陕西杨凌,712100;华中农业大学作物遗传改良国家重点实验室,湖北武汉,430070 2. 西北农林科技大学农学院,陕西杨凌,712100 3. 华中农业大学作物遗传改良国家重点实验室,湖北武汉,430070 |
| 基金项目： | 华中农大作物遗传改良国家重点实验室开放课题项目,"九五"国家重点科技攻关项目. |
| 摘 要： | 从转基因小麦后代分离群体中选择含有外源基因的目标植株是培育转基因小麦的重要环节。用卡那霉素筛选西农1376和西农2611小麦，结果表明，80mg/L卡那霉素能有效抑制非转化小麦幼苗的正常生长；小麦不同基因型对卡那霉素的敏感性稍有差异；经PCR检测，80mg/L卡那霉素筛选出抗性苗的阳性率达到90％。 |
| 关 键 词： | 后代筛选 应用研究 卡那霉素 转基因小麦 |
| 文章编号： | 1004-1389(2002)03-0017-04 |
| 收稿时间： | 2002/1/18 0:00:00 |
| 修稿时间： | 2002年1月18日 |
Application of Kanamycin in the Selection of Transgenic Wheat Progeny |
|
| XI Ya-jun,HOU Wen-sheng,ZHANG Qi-fa and LU Ming.Application of Kanamycin in the Selection of Transgenic Wheat Progeny[J].Acta Agriculturae Boreali-occidentalis Sinica,2002,11(3):17-20. | |
| Authors: | XI Ya-jun HOU Wen-sheng ZHANG Qi-fa and LU Ming |
| Institution: | College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;National Key Lab for Crop Genetics and Improvement of Huazhong Agriculture University, Wuhan Hubei 430070, China;College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China;National Key Lab for Crop Genetics and Improvement of Huazhong Agriculture University, Wuhan Hubei 430070, China;College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling Shaanxi 712100, China |
| Abstract: | Selection target plant with foreign gene from transgenic wheat progeny is the key step to wheat transformation.The selection effects to wheat of Xinong1376 and Xinong2611 with Kanamycin were studied.The results showed that non-transformation wheat could be refrained effectively by Kanamycin of 80 mg/L and there was a littled ifference of different wheat genotype to Kanamycin.By PCR analysis in the resistant seedling to Kanamycin of 80 mg/L,the frequency of PCR positive amounted to 90%. |
| Keywords: | Kanamycin Transgenic wheat Progeny Selection |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《西北农业学报》浏览原始摘要信息 | |
| 点击此处可从《西北农业学报》下载免费的PDF全文 | |