| 甘蓝一代杂种秦甘60及其双亲的同工酶电泳分析 |
| |
| 引用本文： | 王莎莎,张恩慧,马超,许忠民.甘蓝一代杂种秦甘60及其双亲的同工酶电泳分析[J].西北农业学报,2008,17(2):179-182. |
| |
| 作者姓名： | 王莎莎 张恩慧 马超 许忠民 |
| |
| 作者单位： | 1. 西北农林科技大学园艺学院,农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌,712100
2. 贵州省园艺研究所,贵州贵阳,550006 |
| |
| 基金项目： | 国家农业科技成果转化基金资助项目(05EFN217100436)，“十一.五”国家科技支撑计划资助项目(2006BAD01A7-2-04) |
| |
| 摘 要： | 应用同工酶电泳技术,分析了甘蓝一代杂种秦甘60及其亲本叶片中的过氧化物酶(POD)和酯酶同工酶(EST)图谱。结果表明,甘蓝秦甘60(F1)及其亲本在苗期的POD酶谱和EST酶谱表现相一致,都表现为单一亲本型,具有母本的完整酶带。对秦甘60(F1)杂交率的鉴定在其反交种中是可以利用POD酶Rf0.227、0.273酶带或EST酶Rf0.778酶带将其与父本自交种区分开,但在正交种中与母本自交种不易区别。
|
| 关 键 词： | 甘蓝 一代杂种 秦甘60 同工酶 |
| 文章编号： | 1004-1389(2008)02-0179-04 |
| 收稿时间： | 2007/9/28 0:00:00 |
| 修稿时间： | 2007年9月28日 |
Analysis on Isozymes Electrophoresis of Hybrid in Cabbage Qingan60 and Its Parents |
| |
| WANG Sha-sh,ZHANG En-hui,MA Chao and XU Zhong-min.Analysis on Isozymes Electrophoresis of Hybrid in Cabbage Qingan60 and Its Parents[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(2):179-182. |
| |
| Authors: | WANG Sha-sh ZHANG En-hui MA Chao and XU Zhong-min |
| |
| Institution: | College of Horticulturae, Northwest A & F University, Key Laboratory of Horticulture Plant Germplasm Improvement, Ministry of Agriculture, Yangling Shaanxi 712100, China;College of Horticulturae, Northwest A & F University, Key Laboratory of Horticulture Plant Germplasm Improvement, Ministry of Agriculture, Yangling Shaanxi 712100, China;Guizhou Horticulture Institute, Guiyang Guizhou 550006, China;College of Horticulturae, Northwest A & F University, Key Laboratory of Horticulture Plant Germplasm Improvement, Ministry of Agriculture, Yangling Shaanxi 712100, China |
| |
| Abstract: | Applying to isozymes electrophoresis technique,this article analysised the hybrids of cabbage Qingan60 and its parents in seedling stage.The result showed that the hybrids and its parents' POD isoenzyme patterns in leaves were similar to EST isoenzyme patterns.They all have the whole isoenzyme of female plant,all showing the single parent shape.According to the identification of crossing rate of Qingan60(F1),it can be separated the reciprocal crosses hybrids from self-pollinated seeds by using the bands of POD Rf 0.227,0.273 or the bands of EST Rf 0.778.Otherwise orthogonal hybrids are difficult to separate from self-pollinated seeds. |
| |
| Keywords: | Cabbage F1 hybrid Qingan60 Isozyme |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
