| 黄瓜细菌性角斑病菌的分离与鉴定 |
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| 引用本文： | 张吉光,陈 璐,张管曲,胡小平,王保通.黄瓜细菌性角斑病菌的分离与鉴定[J].西北农业学报,2010,19(12):183-187. |
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| 作者姓名： | 张吉光 陈 璐 张管曲 胡小平 王保通 |
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| 作者单位： | 西北农林科技大学,植保学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 高等学校学科创新引智计划(B07049); 教育部新世纪优秀人才支持计划(NCET-07-0698)资助 |
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| 摘 要： | 黄瓜细菌性角斑病是黄瓜生产中的重要病害之一,在陕西的危害严重,发病率在50%以上。本研究采用常规组织分离法和柯赫氏证病律从杨凌地区黄瓜细菌性角斑病标样中分离到1株病原菌,结合形态学、生理生化指标及16S rDNA序列分析,鉴定为丁香假单胞杆菌(Pseudomonas syringestrain)CP001。初步确定适合丁香假单胞杆菌CP001菌株生长的最佳pH为6~8,最适温度为22~29℃。
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| 关 键 词： | 黄瓜细菌性角斑病 丁香假单胞杆菌 病原菌鉴定 16S rDNA |
Isolation and Identification of the Pathogen of Cucumber Angular Leaf Spot |
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| ZHANG Jiguang,CHEN Lu,ZHANG Guanqu,HU Xiaoping and WANG Baotong.Isolation and Identification of the Pathogen of Cucumber Angular Leaf Spot[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(12):183-187. |
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| Authors: | ZHANG Jiguang CHEN Lu ZHANG Guanqu HU Xiaoping and WANG Baotong |
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| Institution: | ZHANG Jiguang,CHEN Lu,ZHANG Guanqu,HU Xiaoping and WANG Baotong(College of Plant Protection,Northwest A&F University,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling Shaanxi 712100,China) |
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| Abstract: | Cucumber angular leaf spot is an important disease in cucumber production in Shaanxi.The incidence of cucumber caused by Pseudomonas syringae was over 50%.Through tissue plating and Koch's pustule methods,one pathogen was isolated from diseased cucumber plant in Yangling of Shaanxi province.The pathogen was identified as Pseudomonas syringe strain CP001 based on morphological characteristics,cultural physiological and chemical characteristics,and the sequence of 16S ribosomal DNA.Biological characteristics ... |
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| Keywords: | Cucumber angular leaf spot Pseudomonas syringae Pathogen identification 16S rDNA |
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