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产黄酮银杏内生菌的分离与鉴定
引用本文:赵庆云,樊明涛,师俊玲.产黄酮银杏内生菌的分离与鉴定[J].西北农业学报,2007,16(2):169-173.
作者姓名:赵庆云  樊明涛  师俊玲
作者单位:西北农林科技大学食品科学与工程学院,陕西杨凌,712100
基金项目:西北农林科技大学校科研和教改项目
摘    要:从健康银杏植株中分离到126株内生菌,并通过HPLC-UV检测方法筛选到8株内生菌产黄酮类物质。用察氏平板培养基分离菌株,根据菌株的菌落形态、大小、颜色、生长速率、质地、生长培养基颜色变化以及菌丝体和孢子的形态特征进行鉴定。初步鉴定结果分别属于匐柄霉、丛梗孢属、交链孢属、镰孢霉属、赤霉属、蜜孢霉、放线菌属和暗梗单孢霉属。

关 键 词:银杏内生菌  黄酮类物质  分离  鉴定
文章编号:1004-1389(2007)02-0169-05
收稿时间:2006-09-14
修稿时间:2006-10-09

Isolation and Identification of Flavones-forming Endophytes from Ginkgo biloba L.
ZHAO Qing-yun,FAN Ming-tao and SHI Jun-ling.Isolation and Identification of Flavones-forming Endophytes from Ginkgo biloba L.[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(2):169-173.
Authors:ZHAO Qing-yun  FAN Ming-tao and SHI Jun-ling
Institution:College of Food Science and Engineering, Northwest A & F University, Yangling Shaanxi 712100, China;College of Food Science and Engineering, Northwest A & F University, Yangling Shaanxi 712100, China;College of Food Science and Engineering, Northwest A & F University, Yangling Shaanxi 712100, China
Abstract:In this paper,the 126 endophytes from healthy Ginkgo biloba L.were isolated and screened eight strains produced flavonoids substances by HPLC-UV.Their morphological and physiological characteristics were observed through the strains colony morphology,size,color,growth rate,texture,and spores.The results showed that they are Stemphylium sp,Fusella Sacc,Alternaria sp,Fusarium sp,Gibberella sp,Sphacelia sp,Actinomyces,Dematium Pers.
Keywords:Endophytes of Ginkgo biloba L  Flavones  Isolation  Identification
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