| 加工番茄离体再生体系的建立 |
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| 引用本文： | 陈丽萍,张丽华,程智慧.加工番茄离体再生体系的建立[J].西北农业学报,2007,16(1):162-167. |
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| 作者姓名： | 陈丽萍 张丽华 程智慧 |
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| 作者单位： | 西北农林科技大学园艺学院,陕西杨凌,712100 |
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| 基金项目： | 新疆生产建设兵团基金;国家自然科学基金 |
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| 摘 要： | 以加工番茄B04子叶盘和下胚轴切段为外植体,研究了不同种类和浓度的细胞分裂素(ZT、6-BA和KT)和生长素(IAAI、BA和NAA)组合及附加物AgNO3对外植体不定芽诱导的影响。结果表明,细胞分裂素ZT的不定芽诱导效果明显优于6-BA和KT,KT诱导效果最差;生长素IAA的不定芽诱导效果优于IBA和NAA;在不同的激素组合中,不定芽诱导率最高的培养基为MS 2.0 mg/L ZT 0.2 mg/L IAA。再生芽在MS 0.1mg/L IAA生根培养基上能正常生根,并发育成完整的小植株。
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| 关 键 词： | 加工番茄 再生体系 子叶 下胚轴 |
| 文章编号： | 1004-1389（2007）01-0162-06 |
| 收稿时间： | 2006-07-11 |
| 修稿时间： | 2006-08-10 |
Establishment of in vitro Regeneration System for Processing Tomato |
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| CHEN Li-ping,ZHANG Li-hua and CHENG Zhi-hui.Establishment of in vitro Regeneration System for Processing Tomato[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(1):162-167. |
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| Authors: | CHEN Li-ping ZHANG Li-hua and CHENG Zhi-hui |
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| Institution: | College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | Cotyledons and hypocotyls of processing tomato cv.B04 were used as explants to study different combinations of cytokinin(ZT,6-BA or KT) with auxin(IAA,IBA or NAA) and silver nitrate on bud induction and sensitivity to kanamycin.The results showed that the effect of ZT was better than that of 6-BA or KT,and IAA was better than IBA or NAA on bud induction.The best medium on bud induction was MS ZT 2.0 mg/L IAA 0.2 mg/L among different combinations.The regenerated buds could develop normally after roots were induced on MS IAA 0.1 mg/L. |
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| Keywords: | Processing tomato Regeneration system Cotyledon Hypocotyls |
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