气质联用法(GC-MS)检测禾谷镰刀菌代谢指纹分析

代谢指纹分析(Metabolic fingerprint analysis)是代谢组学研究的重要分析模式,广泛应用于各类动植物体的非靶标的代谢产物的探索研究中,其在真菌微生物方面的研究却很少。采用气质联用法(GC-MS)对衍生化后的禾谷镰刀菌样品进行指纹图谱分析,比较不同培养时间、培养环境、不同衍生化方法对代谢产物的影响,选择最佳条件,鉴定出63种代谢产物。这为真菌类物质的代谢组学研究提供了参考依据。

Metabolite Fingerprinting Analysis of Fusarium graminearum by Gas Chromatography-Mass Spectrometry(GC-MS)

Metabolic fingerprint analysis is an important analytical method for metabolomics research,it is widely used in various animal and plant organisms for exploration of non-target metabolites,but it is rarely reported to be used in fungi. In this paper,a metabolic fingerprint analytical method for fungi was established by using gas chromatography-mass spectrometry(GC-MS) through analyzing the fingerprints of the derivatized Fusarium graminearum samples. The culture periods,culture environment and derivatization methods in kinds of metabolites were initially screened. Under the optimal condition with the most numbers of metabolites,63 metabolites were identified. This study provides an effective analytical method for the metabolomics study of fungi.