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PCV2、PPV、PRV、PRRSV和CSFV复合PCR的应用研究
引用本文:李维华,任慧英,刘文华,邹玲,温建新,李海忠.PCV2、PPV、PRV、PRRSV和CSFV复合PCR的应用研究[J].西北农业学报,2008,17(3):12-15.
作者姓名:李维华  任慧英  刘文华  邹玲  温建新  李海忠
作者单位:青岛农业大学,动物科技学院,山东青岛,266109
基金项目:山东省青岛市科技攻关项目
摘    要:采用建立的PCV2-PPV-PRV-PRRSV-CSFV复合PCR检测方法,对山东省不同地区送检的32份临床疑似病料进行PCR检测。结果表明:32份样品中,PCV2、PPV、PRV、PRRSV和CSFV感染的阳性率分别为25.00%、0%、34.38%、71.88%和3.13%;共检出25份阳性病料,阳性率为78.13%,其中PRV-PRRSV双重感染的比例为31.25%,PCV2-PRV-PRRSV三种病原体混合感染的比例为25.00%;用单项PCR检测作对照,结果两者符合率为100%,表明该复合PCR检测方法具有较高的敏感性,可以作为临床上猪圆环病毒2型感染、猪细小病毒病、猪伪狂犬病、猪繁殖与呼吸综合征和猪瘟的病原学快速诊断方法。

关 键 词:猪圆环病毒2型  猪细小病毒  猪伪狂犬病毒  猪繁殖与呼吸综合征病毒  猪瘟病毒  复合PCR
收稿时间:2007/10/26 0:00:00
修稿时间:2008/1/10 0:00:00

Application of the Multiplex PCR Detecting PCV2, PPV, PRV, PRRSV and CSFV
LI Wei-hu,REN Hui-ying,LIU Wen-hu,ZOU Ling,WEN Jian-xin and LI Hai-zhong.Application of the Multiplex PCR Detecting PCV2, PPV, PRV, PRRSV and CSFV[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(3):12-15.
Authors:LI Wei-hu  REN Hui-ying  LIU Wen-hu  ZOU Ling  WEN Jian-xin and LI Hai-zhong
Institution:College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China;College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China;College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China;College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China;College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China;College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China
Abstract:The established multiplex PCR of PCV2-PPV-PRV-PRRSV-CSFV was used to detect thirty-two clinical cases.These cases were collected from different areas of Shandong Province for the PCR detection.The results showed that the infection rate of PCV2,PPV,PRV,PRRSV and CSFV was 25.00%,0%,34.38%,71.88% and 3.13%,respectively.Twenty-five positive cases were detected and positive rate was 78.13%.The infection ratio of PRV-PRRSV double pathogens was 31.25% and the infection ratio of PCV2-PRV-PRRSV three pathogens was 25.00%.The single PCR was used as a control,and the result showed that the multiplex PCR accorded with the single PCR about 100%.It indicated the multiplex PCR had higher sensitivity.The multiplex PCR can be used as a rapid etiology diagnostic method to detect clinical diseases of porcine circovirus type 2 infection,porcine parvovirus disease,swine pseudorabies,porcine reproductive and respiratory syndrome and classical swine fever.
Keywords:Porcine circovirus type 2  Porcine parvovirus  Pseudorabies virus  Porcine reproductive and respiratory syndrome virus  Classical swine fever virus  Multiplex PCR
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