| 抗坏血酸过氧化物酶基因转化苹果的研究 |
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| 引用本文： | 李 颖,马锋旺.抗坏血酸过氧化物酶基因转化苹果的研究[J].西北农业学报,2010,19(1):140-143. |
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| 作者姓名： | 李 颖 马锋旺 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西杨凌,712100;西藏自治区农科院,中心实验室,拉萨,850002
2. 西北农林科技大学,园艺学院,陕西杨凌,712100 |
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| 基金项目： | 西北农林科技大学 拔尖人才支持计划 项目 |
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| 摘 要： | 利用根癌农杆菌介导法对"嘎啦"苹果叶片进行遗传转化,获得"嘎啦"苹果的转基因植株.2 d的预培养,OD600值约为0.6 的工程菌液浓度,侵染时间10 min,3 d的共培养,延迟筛选10 d获得较高的转化效率.PCR检测和Gus染色表明,APX基因已整合到苹果基因组中.
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| 关 键 词： | 苹果 根癌农杆菌 遗传转化 抗坏血酸过氧化物酶基因 |
| 收稿时间： | 2009/8/12 0:00:00 |
| 修稿时间： | 2009/9/10 0:00:00 |
Agrobacterium-mediated Transformation of Gala Apple with APX Gene |
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| LI Ying and MA Fengwang.Agrobacterium-mediated Transformation of Gala Apple with APX Gene[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(1):140-143. |
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| Authors: | LI Ying and MA Fengwang |
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| Institution: | LI Ying1,2 , MA Fengwang1(1.College of Horticulture,Northwest A&F University,Yangling Shaanxi 712100,China,2.Central Laboratory of the Tibet Autonomous Regional Academy of Agricultural Sciences,Lasa 850002,China) |
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| Abstract: | The transgenic Gala apple plants with APX gene by Agrobacterium tumefacien were obtained.The results showed that highest transformation frequency was obtained when the leaves was precultivated 2 days,the OD600 of Agrobacterium was about 0.6,the Agrobacterium infected 10 min,3 days coculture,and 10 days of detention screening.Results of PCR and Gus proved that the APX gene had integrated into apple genome. |
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| Keywords: | Apple Agrobacterium tumefacien Genetic transformation Ascorbate peroxidase gene |
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