| 糯玉米DNA提取方法的优化及SSAP方法的建立 |
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| 引用本文： | 马小锋,段可,胡景江.糯玉米DNA提取方法的优化及SSAP方法的建立[J].西北农业学报,2008,17(6):73-78. |
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| 作者姓名： | 马小锋 段可 胡景江 |
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| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西杨凌,712100
2. 上海市农业科学院生物技术研究所,上海,201106 |
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| 基金项目： | 上海市科学技术委员会资助项目
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| 摘 要： | 在综合现有DNA提取方法和试验材料本身性质的基础上,对糯玉米基因组DNA提取方法进行了优化和改进,筛选出一种适合于糯玉米特异序列扩增多态性(sequence-specific amplification polymorphisms,SSAP)分析的高纯度DNA提取方法。所得DNA的A260/280在1.8~2.0间,A260/230大于2.0,琼脂糖凝胶电泳检测结果也证明该方法获得的DNA完整、无降解现象。通过对不同酶切组合、引物组合等因素的对比试验,初步建立了适用于糯玉米SSAP分析的方法体系,得到了一组条带清晰和多态性丰富的图谱。
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| 关 键 词： | 糯玉米 DNA 提取 |
| 收稿时间： | 2008/7/15 0:00:00 |
| 修稿时间： | 2008/8/2 0:00:00 |
DNA Extraction Optimization and Establishment of SSAP on Waxy Corn |
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| MA Xiao-feng,DUAN Ke and HU Jing-jiang.DNA Extraction Optimization and Establishment of SSAP on Waxy Corn[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(6):73-78. |
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| Authors: | MA Xiao-feng DUAN Ke and HU Jing-jiang |
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| Institution: | College of Life Science, Northwest A&F University Yangling Shaanxi 712100, China;The Biotechnology Research Center Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;College of Life Science, Northwest A&F University Yangling Shaanxi 712100, China |
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| Abstract: | Base on the existed DNA extraction methods and the nature of waxy corn,waxy corn genome DNA extraction method has been optimized and improved.A260/280 of the DNA obtained by the method is between 1.8 and 2.0,and A260/230 is more than 2.0.The test result of agarose gel electrophoresis have proved that the DNA is compiete.it is more suitable for sequencespecific amplification polymorphisms(SSAP) analysis.Through comparative experiments,such as the combination of different enzymes restriction,different primers combination,SSAP analysis has been established on waxy corn,Clear bands and high polymorphisms have been got.The research made a foundation of germplasm,genetic diversity and fine breeding for waxy corn. |
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| Keywords: | SSAP |
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