| E2、P4、OT和PGF2α对猪PBMC活性的影响 |
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| 引用本文： | 吴瑞,楚元奎,丰艳妮,吴庆侠,靳亚平.E2、P4、OT和PGF2α对猪PBMC活性的影响[J].西北农业学报,2007,16(4):52-54,66. |
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| 作者姓名： | 吴瑞 楚元奎 丰艳妮 吴庆侠 靳亚平 |
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| 作者单位： | 西北农林科技大学动物科技学院,农业部家畜生殖内分泌与胚胎工程重点开放实验室,陕西杨凌712100 |
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| 基金项目： | 国家自然科学基金资助项目（39770545）;西北农林科技大学青年骨干教师支持计划项目（01140305）. |
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| 摘 要： | 应用MTT比色法，以四因素四水平L16（4^5）的正交试验测定E2、P4、OT和PGF2a四种激素对猪的外周血单个核细胞（PBMC）的转化效果。结果显示，所选的4种激素在不同浓度组合时刺激PBMC转化的作用不同。这4种激素对PBMC的转化影响的强度依次为：PGFza、OT、E2、P4。在试验剂量范围内，E2 0.1Pg／mL、P41 ng／mL、OT 10 pg／mL、PGF2a 100 ng／mL时，其共同作用对PBMC转化活性最强。提示在不同生理时期，由于机体内各种激素浓度变化不同，免疫系统也发生相应的变化。
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| 关 键 词： | 激素 淋巴细胞 正交试验 |
| 文章编号： | 1004-1389（2007）04-0052-03 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006-05-222007-01-20 |
Activation of Pig PBMC Induced by E2, P4,OT and PGF2a |
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| WU Rui,CHU Yuan-kui,FENG Yan-ni,WU Qing-xia and JIN Ya-ping.Activation of Pig PBMC Induced by E2, P4,OT and PGF2a[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(4):52-54,66. |
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| Authors: | WU Rui CHU Yuan-kui FENG Yan-ni WU Qing-xia and JIN Ya-ping |
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| Institution: | Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;Key laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology, Ministry of Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | |
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| Keywords: | Hormone Leukomonocyte Orthogonal design |
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