茶氨酸衍生物茶溴香酰胺脂质体对人肺癌细胞 生长和迁移的抑制作用

为了探索研究茶氨酸衍生物茶溴香酰胺制备的脂质体(TBrC-L)对人非小细胞肺癌A549细胞生长和迁移的抑制作用与其分子机制,采用薄膜分散法制备了TBrC-L,通过MTT法检测不同浓度的TBrC-L对人肺癌A549细胞生长的抑制作用;使用流式细胞术检测TBrC-L对人肺癌A549细胞凋亡的诱导作用;利用Transwell chamber法观察TBrC-L对人肺癌A549细胞迁移作用的影响;应用蛋白质印迹法检测人肺癌A549细胞中与凋亡和生长密切相关蛋白的表达和药物可能的作用靶点。实验结果显示,TBrC-L对人肺癌A549细胞生长和迁移有显著的抑制作用,促进肿瘤细胞凋亡、抑制肿瘤细胞生长和迁移可能是其抗人肺癌作用的重要机制,其作用的分子机制涉及到抑制EGFR、VEGFR1、VEGFR~2和Met受体介导的Akt和NF-κB信号传导通路,本研究结果提示,TBrC-L具有应用于临床治疗和(或)辅助治疗人肺癌的潜力。

Inhibitory effects of theanine derivative TBrC-prepared liposome on the growth and migration of human lung cancer cells

This study is to investigate the inhibitory effects of theanine derivative TBrC-prepared liposome (TBrC-L) on the growth and migration of human non-small-cell lung cancer (NSCLC) A549 cells and its molecular mechanism.The MTT assay and flow cytometry were used to detect the effects of different concentrations of TBrC-L on the growth and apoptosis of human lung cancer A549 cells,respectively.The transwell chamber assay and westem blot were applied to determining the effects of TBrC-L on the cancer cell migration and related protein expressions,respectively.The results showed that TBrC-L significantly inhibited the cancer cell growth and migration and induced apoptosis in human lung cancer A549 cells.The suppression of the cancer cell growth and migration as well as the induction of apoptosis may be the important anticancer activities of TBrC-L against the human lung cancer A549 cells.The molecular mechanism of TBrC-L action may be involved in restraining the EGFR-,VEGFR1-,VEGFR2-and Met-mediated signaling transduction pathways of Akt and NF-κB in the human cancer cells.The results suggested that TBrC-L may have the potential to be used in clinical treatment and/or adjuvant treatment of human non-small-cell lung cancer.