| 茶树的RAPD标记向SCAR标记转化的研究 |
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| 引用本文： | 丁洲,江昌俊,叶爱华,陈聪,谭振,张莉.茶树的RAPD标记向SCAR标记转化的研究[J].安徽农业大学学报,2008,35(3). |
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| 作者姓名： | 丁洲 江昌俊 叶爱华 陈聪 谭振 张莉 |
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| 作者单位： | 安徽农业大学生命科学学院,合肥,230036;安徽农业大学茶与食品科技学院,合肥,230036 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | SCAR标记是在RAPD技术的基础上发展起来的一种新型分子标记技术,相对其他分子标记,它具有开发成本低,稳定性高,单位点多态等特点,将为茶树分子育种开辟一条新的有效途径。通过将随机引物在不同茶树品种基因组DNA中扩增得到的特异性片段,经回收、克隆、测序后重新设计1对特异性引物,将RAPD标记成功转化成SCAR标记,提高了分子鉴定的稳定性。
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| 关 键 词： | 茶树 RAPD标记 SCAR标记 转化 |
Research on RAPD marker to SCAR marker in Camellia sinensis |
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| DING Zhou,JIANG Chang-jun,YE Ai-hu,CHEN Cong,TAN Zhen,ZHANG Li.Research on RAPD marker to SCAR marker in Camellia sinensis[J].Journal of Anhui Agricultural University,2008,35(3). |
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| Authors: | DING Zhou JIANG Chang-jun YE Ai-hu CHEN Cong TAN Zhen ZHANG Li |
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| Abstract: | SCAR marker is one of new molecular markers based on RAPD technology.It has such merit as low price,high stability,site-specific and so on compared with other markers,and it may provide a new way for molecular breeding of tea.In this experiment,the special segment with random primer in different tea cultivars genomic DNA was recovered,cloned and sequenced.According to its sequence,a pair of special primers was synthesized and the RAPD marker was successfully converted into SCAR marker.It improves stability of molecular identification and affirm applied prospect of SCAR technology in genetic resources of tea. |
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| Keywords: | tea plant(Camellia sinensis) RAPD marker SCAR marker convert |
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