| 北京荷斯坦母牛7个微卫星基因座遗传多态性研究 |
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| 引用本文： | 周国利,储明星,金海国,石万海,曹福存,朱颜. 北京荷斯坦母牛7个微卫星基因座遗传多态性研究[J]. 安徽农业大学学报, 2005, 32(3): 293-297 |
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| 作者姓名： | 周国利 储明星 金海国 石万海 曹福存 朱颜 |
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| 作者单位： | 聊城大学生物系,聊城,252059;中国农业科学院畜牧研究所,北京,100094;延边大学农学院动物科学系,龙井,133400;北京奶牛中心,北京,100085 |
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| 摘 要： | 利用7个微卫星基因座BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21对240头北京荷斯坦母牛进行遗传检测,用非变性(中性)聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物.结果表明BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21的等位基因数/多态信息含量/有效等位基因数/杂合度分别为4/0.3869/1.7693/0.4348、5/0.5923/2.9121/0.6566、8/0.7114/3.9012/0.7437、6/0.5921/2.8244/0.6459、6/0.7122/3.9989/0.7499、6/0.7144/4.0124/0.7508和7/0.6154/2.9805/0.6645.可见BM4505的遗传变异最大,BM1818的遗传变异最小.
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| 关 键 词： | 北京荷斯坦母牛 微卫星基因座 遗传多态性 |
| 文章编号： | 1672-352X(2005)03-0293-05 |
| 修稿时间： | 2004-05-28 |
Genetic Polymorphisms of Seven Microsatellite Loci in Beijing Holstein Cows |
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| ZHOU Guo-li,CHU Ming-xing,JIN Hai-guo,SHI Wan-hai,CAO Fu-cun,ZHU Yan. Genetic Polymorphisms of Seven Microsatellite Loci in Beijing Holstein Cows[J]. Journal of Anhui Agricultural University, 2005, 32(3): 293-297 |
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| Authors: | ZHOU Guo-li CHU Ming-xing JIN Hai-guo SHI Wan-hai CAO Fu-cun ZHU Yan |
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| Abstract: | The polymorphisms of seven microsatellite loci BM1818, BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were detected in 240 Beijing Holstein cows.The PCR amplified products of microsatellites were detected by non-denatured (neutral) polyacrylamide gel electrophoresis. The number of alleles/polymorphic information content/ effective numbers of alleles/heterozygosity of BM1818,BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were 4/0.3869/1.7693/0.4348, 5/0.5923/2.9121/0.6566, 8/0.7114/3.9012/0.7437, 6/0.5921/2.8244/0.6459, 6/0.7122/3.9989/0.7499, 6/0.7144/4.0124/0.7508 and 7/0.6154/2.9805/0.6645, respectively. The results revealed the greatest genetic variation in BM4505 and the lowest in BM1818. |
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| Keywords: | Beijing Holstein cows microsatellite loci genetic polymorphisms |
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