| 一个毛竹细胞色素P450基因的克隆与表达研究 |
| |
| 引用本文： | 杨学文,彭镇华.一个毛竹细胞色素P450基因的克隆与表达研究[J].安徽农业大学学报,2010,37(1):116-121. |
| |
| 作者姓名： | 杨学文 彭镇华 |
| |
| 作者单位： | 国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京,100102;国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京,100102;中国林业科学研究院林业研究所,北京,100091 |
| |
| 基金项目： | 林业公益性行业科研专项项目(200704001);;国家林业局重点项目(2007-01)共同资助 |
| |
| 摘 要： | 从毛竹全长cDNA文库中分离到1个细胞色素P450基因,命名为PheCYP-1。相似性分析表明,其编码蛋白与拟南芥CYP706A亚家族蛋白相似性较高(42%~46%),属于CYP706家族;实时定量PCR结果表明,PheCYP-1在正在伸长的笋中表达丰度最高;将PheCYP-1构建到pBI121的多克隆位点,在拟南芥中过量表达,转基因拟南芥的次生木质部导管壁厚度显著增加,表明PheCYP-1基因可能与毛竹次生木质部的细胞壁发育有关。
|
| 关 键 词： | 毛竹 细胞色素P450 木质素 细胞壁 转基因 |
Cloning and expression of a cytochrome P450 gene from moso bamboo |
| |
| YANG Xue-wen,PENG Zhen-hua.Cloning and expression of a cytochrome P450 gene from moso bamboo[J].Journal of Anhui Agricultural University,2010,37(1):116-121. |
| |
| Authors: | YANG Xue-wen PENG Zhen-hua |
| |
| Institution: | 1.SFA Key Laboratory of Bamboo and Rattan Science and Technology;International Center for Bamboo and Rattan;Beijing 100102;2.Research Institute of Forestry;Chinese Academy of Forestry;Beijing 100091 |
| |
| Abstract: | A gene encoding cytochrome P450 protein was isolated from moso bamboo(Phyllostachys edulis) full-length cDNA library,and designated as PheCYP-1.BLASTp analysis showed that its deduced amino acid sequence had relatively high similarity to CYP706A subfamily proteins from Arabidopsis thaliana(42% to 46%),which indicated it belongs to CYP706 family.Quanitative real-time PCR results showed that it was mainly expressed in elongating shoots of P.edulis.PheCYP-1 was cloned into multiple clone sites of pBI121 and tr... |
| |
| Keywords: | Phyllostachys edulis cytochrome P450 xylem cell wall transgene |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《安徽农业大学学报》浏览原始摘要信息 |
| 点击此处可从《安徽农业大学学报》下载免费的PDF全文 |
