猕猴桃体外快繁条件优化及高效再生体系的建立

为进一步优化‘红阳’猕猴桃（Actinidia chinensis cv. Hongyang）组织培养育苗技术体系，采用‘红阳’猕猴桃无菌组培苗的茎尖和叶片为材料，以ZT 1.5 mg·L^-1为外源激素，筛选适宜的基本培养基，并研究不同外源激素及组合对‘红阳’猕猴桃间接、直接器官发生及植株再生的影响。结果表明，适合‘红阳’猕猴桃生长的基本培养基为OM。在OM + 2.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 0.01 mg·L^-1 2, 4-D培养基中，叶片愈伤诱导率为100%，不定芽发生系数为3.68；而带叶茎尖在OM + 1.5 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 1.0 mg·L^-1 KT培养基中培养60 d后，通过直接器官产生基茎丛生芽，其增殖系数可达7.65。试管苗适宜的生根培养基为1/2 OM + 0.5 mg·L^-1 NAA，60 d后平均不定根数为4.87，驯化移栽后成活率为90%以上。选择出了适宜‘红阳’猕猴桃生长的基本培养基，解决了组培苗叶片黄化、植株矮小和畸形的问题，且大幅提高了增殖系数。优化了‘红阳’猕猴桃的人工快繁体系，可为猕猴桃其他品种的研究提供参考。

Optimization of rapid propagation conditions and establishment of an efficient regeneration system for kiwifruit in vitro

In order to further optimize the technical system of tissue culture and seedling raising of Actinidia chinensis cv. Hongyang, the stem tips and leaves of sterile tissue culture seedlings of A. chinensis cv. Hongyang were used as the materials, and ZT 1.5 mg·L^-1 was used as the plant growth regulator to screen a suitable basic medium, and then the effects of different combinations of plant growth regulators on indirect and direct organogenesis and plant regeneration were studied. The results showed that: the optimum medium for the growth of A. chinensis cv. Hongyang was OM. In the medium of OM + 2.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 0.01 mg·L^-1 2, 4-D, the callus induction rate was 100%, and the adventitious bud generation coefficient was 3.68; the stem tips were cultured in OM + 1.5 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 1.0 mg·L^-1 KT for 60 days, the basal stem cluster buds were produced by direct organogenesis, and the proliferation coefficient could reach 7.65. The optimal rooting medium for test-tube seedlings was 1/2 OM+NAA 0.5 mg·L^-1; after 60 days, the average number of adventitious roots was 4.87, and the survival rate of domesticated seedlings was more than 90%. The basic medium suitable for the growth of A. chinensis cv. Hongyang was selected, and the problems of leaf yellowing, dwarf and deformity of test-tube plantlets were solved, and the proliferation coefficient was greatly increased. The artificial rapid propagation system of A. chinensis cv. Hongyang was optimized to provide a reference for the research of other kiwifruit varieties.