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银杏叶提取物对体外培养的肉仔鸡脾脏淋巴细胞 增殖及分泌IL-2的影响
引用本文:黄其春,杨小燕,林淑慧,何玉琴,李焰.银杏叶提取物对体外培养的肉仔鸡脾脏淋巴细胞 增殖及分泌IL-2的影响[J].安徽农业大学学报,2012,39(3):348-351.
作者姓名:黄其春  杨小燕  林淑慧  何玉琴  李焰
作者单位:龙岩学院生命科学学院,龙岩364000;预防兽医学与生物技术福建省高等学校重点实验室,龙岩364000;龙岩学院生命科学学院,龙岩364000;福建省人畜寄生与病毒性疫病防控工程技术中心,龙岩364000;福建农林大学动物科学学院,福州,350002
基金项目:福建省科技计划重点项目(2007N0049)资助
摘    要:为研究银杏叶提取物(EGB)对体外培养的肉仔鸡脾脏淋巴细胞增殖及分泌白介素-2(IL-2)的影响,将不同质量浓度的EGB分别加入到体外培养的由伴刀豆球蛋白A(ConA)诱导的肉仔鸡脾脏淋巴细胞中,培养24 h、48 h和72 h后,再分别采用MTT法、ELISA法测定淋巴细胞增殖的变化和IL-2的分泌量。结果显示,质量浓度为3.33、6.67、13.33、20.00、26.67和33.33 mg.mL-1的EGB能显著促进由ConA诱导的肉仔鸡脾脏淋巴细胞增殖和分泌IL-2(P0.05),其中质量浓度为20.00 mg.mL-1的EGB作用效果较好,表明EGB在体外能促进由ConA诱导的肉仔鸡脾脏淋巴细胞增殖和分泌IL-2。

关 键 词:银杏叶提取物  肉仔鸡  脾脏淋巴细胞  细胞增殖  IL-2

Effects of Ginkgo biloba extract on proliferation and IL-2 excretion of broiler spleen lymphocytes cultured in vitro
HUANG Qi-chun,YANG Xiao-yan,LIN Shu-hui,HE Yu-qin and LI Yan.Effects of Ginkgo biloba extract on proliferation and IL-2 excretion of broiler spleen lymphocytes cultured in vitro[J].Journal of Anhui Agricultural University,2012,39(3):348-351.
Authors:HUANG Qi-chun  YANG Xiao-yan  LIN Shu-hui  HE Yu-qin and LI Yan
Institution:1,2(1.College of Life Science,Longyan University,Longyan 364000; 2.Key Laboratory of Fujian Universities Preventive Veterinary Medicine and Biotechnology,Longyan 364000; 3.Fujian Provincial Engineering Technology Center of Prevention and Control in Zoonotic Parasite and Viral Livestock Diseases,Longyan 364000; 4.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002)
Abstract:An experiment was conducted to investigate the effects of Ginkgo biloba extract(EGB) on prolif-eration and interleukin-2(IL-2) excretion of broiler spleen lymphocyte cultured in vitro.Different concentrations of EGB were added into ConA-induced broiler spleen lymphocytes which were cultured in vitro.After 24 hours,48 hours and 72 hours of culture,lymphocyte proliferation and IL-2 excretion were determined by MTT colori-metric assay and ELISA,respectively.The results showed that EGB with concentrations of 3.33,6.67,13.33,20.00,26.67 and 33.33 mg?mL-1 could stimulate ConA-induced proliferation and IL-2 excretion of broiler spleen lymphocyte significantly(P<0.05).The effect of EGB with the concentration of 20.00 mg?mL-1 was better than that of EGB with other concentrations.The study suggested that EGB could stimulate ConA-induced proliferation and IL-2 excretion of broiler spleen lymphocyte in vitro.
Keywords:Ginkgo biloba extract  broiler  spleen lymphocyte  proliferation  IL-2
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