| 5个山羊品种PRKAG3基因5’调控区和外显子13杂合度分析 |
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| 引用本文： | 靳海,陈宏权,黄生强,秦婕,任春环,朱银剑,焦明慧,潘中婷,谢亚男,陈公伟,储明星.5个山羊品种PRKAG3基因5’调控区和外显子13杂合度分析[J].安徽农业大学学报,2012,39(2):165-169. |
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| 作者姓名： | 靳海 陈宏权 黄生强 秦婕 任春环 朱银剑 焦明慧 潘中婷 谢亚男 陈公伟 储明星 |
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| 作者单位： | 湖南农业大学动物科技学院,长沙410128;安徽农业大学动物科技学院,合肥230036;安徽农业大学动物科技学院,合肥230036;安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥230036;湖南农业大学动物科技学院,长沙,410128;安徽农业大学动物科技学院,合肥,230036;中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 国家现代肉羊产业技术体系(nycytx-39);安徽省现代肉羊产业技术体系基金;农业部转基因生物新品种培育重大专项(2009ZX08008-007B)共同资助 |
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| 摘 要： | 以3个中国地方山羊品种和2个引入山羊品种为研究对象,采用PCR-RFLP方法检测山羊PRKAG3基因5’调控区和外显子13的多态性。结果在5个山羊品种PRKAG3基因的5’调控区发现C-525A和C-225T多态位点,在外显子13发现T90C和C102T多态位点,分别存在一对等位基因。5个山羊品种在C-525A、C-225T和C102T位点均为低度多态,但在T90C位点存在中度多态(0.25
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| 关 键 词： | PRKAG3基因 多态性 山羊 肉质 |
The heterozygosity in the 5 ' regulatory region and exon 13 of PRKAG3 gene in five goat breeds |
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| JIN Hai,CHEN Hong-quan,HUANG Sheng-qiang,QIN Jie,REN Chun-huan,ZHU Yin-jian,JIAO Ming-hui,PAN Zhong-ting,XIE Ya-nan,CHEN Gong-wei and CHU Ming-xing.The heterozygosity in the 5 '' regulatory region and exon 13 of PRKAG3 gene in five goat breeds[J].Journal of Anhui Agricultural University,2012,39(2):165-169. |
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| Authors: | JIN Hai CHEN Hong-quan HUANG Sheng-qiang QIN Jie REN Chun-huan ZHU Yin-jian JIAO Ming-hui PAN Zhong-ting XIE Ya-nan CHEN Gong-wei and CHU Ming-xing |
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| Institution: | 1.School of Animal Science and Technology,Hunan Agricultural University,Changsha 410128; 2.School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036; 3.Local Animal Genetic Resources Conservation and Bio-breeding Laboratory of Anhui Province,Hefei,230036; 4.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Science, Chinese Academy of Agricultural Sciences,Beijing 100193) |
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| Abstract: | The three Chinese indigenous goat breeds,Anhui white goat(Anbai),Xiangdong black goat(Xiangdong) and Matou,and two imported goat breeds,Boer and Sanen,were selected.The polymorphisms in the 5’ regulatory region and exon 13 of PRKAG3 gene were detected using PCR-RFLP and estimated via heterozy-gosity and polymorphism information content(PIC).The results showed that four polymorphic loci,C-525A and C-225T in the 5’ regulatory region and T90C and C102T in the exon 13,were found in five goat breeds.The C-525A,C-225T and C102T were low-grade polymorphic loci,but the T90C was moderate polymorphic locus(0.25
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| Keywords: | PRKAG3 gene polymorphism goat meat quality |
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