一株耐盐菌的分离鉴定及其孔雀石绿脱色的研究

从舟山双峰盐场的盐田中分离筛选到一株对孔雀石绿具有较强脱色能力的细菌,经鉴定为腐生葡萄球菌(Staphylococcus saprophyticus sp. JJ-1)。研究孔雀石绿的浓度、脱色pH、氯化钠浓度及氧气对染料脱色的影响,并对脱色产物进行紫外-可见吸收光谱分析,HPLC分析和GC-MS分析,以揭示孔雀石绿的降解产物。脱色反应条件初步研究表明,Staphylococcus saprophyticus sp. JJ-1在4 h内对200 mg·L-1孔雀石绿脱色率可高达95%;该菌在低浓度孔雀石绿(50 mg·L-1)时能够在1 h内快速脱色;在有氧和厌氧条件下该菌的脱色率基本保持一致;在15%NaCl,200mg·L-1孔雀石绿条件下,5 h后的脱色率为94%;该菌脱色的适宜p H 7～10,培养时间4 h,脱色率达到95%。孔雀石绿的主要降解产物为4-(二甲氨基)二苯甲酮。

Study on decolorization of malachite green by a Staphylococcus saprophyticus

One bacterial strain, with excellent capability of malachite green decolorization, was isolated from salt pans of Zhoushan Shuangfeng saltern. This strain was identified as Staphylococcus saprophyticus sp. JJ-1. The effect of various parameters (concentration of malachite green, pH, concentration of NaCl and oxygen) on the removal of malachite green was evaluated. Decolorization products were analyzed by UV-visible analysis, HPLC analysis and GC-MS analysis. The results showed that the decolorization efficiency was up to 95% after 4 h when malachite green concentration was 200 mg·L^-1. The malachite green with low concentration (<50 mg·L^-1) was rapidly decolorized within 1 h. There is no significant difference of decolorization efficiency between aerobic and anaerobic conditions. The decolorization efficiency of 200 mg·L^-1 malachite green after 5 h was 94% in the presence of 15% NaCl, the decolorization efficiency was high between pH 7-10, which reached 95% after 4h. The degradation products of malachite green was 4-(dimethylamino) benzophenone.