| 以关联分析发掘小麦抗除草剂基因位点及其优异等位类型 |
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| 引用本文： | 常成,李仁汉,吴琼,郑标,任凡凡,方冬冬,朱玉磊,王升星,刘凯,陈雪健,张海萍.以关联分析发掘小麦抗除草剂基因位点及其优异等位类型[J].安徽农业大学学报,2019,46(1):104-110. |
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| 作者姓名： | 常成 李仁汉 吴琼 郑标 任凡凡 方冬冬 朱玉磊 王升星 刘凯 陈雪健 张海萍 |
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| 作者单位： | 安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036;安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,合肥,230036 |
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| 基金项目： | 2017年安徽省大学生创新创业计划项目“抗除草剂小麦品种的筛选及其抗性关联分析（201710364075）”和国家卓越农林人才教育培养计划改革试点项目“复合应用型人才培养”共同资助。 |
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| 摘 要： | 利用分布于小麦全基因组的369对SSR分子标记,分析98份育种亲本材料的基因型,并采用TASSLE软件的GLM和MLM模型鉴定除草剂抗性紧密关联的标记位点、发掘关联标记的优异等位类型。以2,4-滴丁酯处理条件下的芽鲜重抑制率(T1)、甲基二磺隆处理下的芽鲜重抑制率(T2)和根鲜重抑制率(T3)等3个指标为目标性状,共关联到37个显著标记位点(P0.05),分布于小麦染色体1AL、1BL、1DL、2AS、2BS、3AL、3BL、3BS、4AL、4BL、5AL、5DL、5DS、6BL、6DL、6DS、7AL、7BL、7BS、7DS和7DL上。其中3BS和4BL上的分子标记Xgwm285和Xgwm165同时在3个指标中均关联到,属于稳定的抗性位点;另有4个标记位点(Xcfd32、Xwmc617、Xmag1263和Xmag2137)同时在2个指标(T2和T3)关联到。优异等位类型分析表明,3个指标均重复检测到的Xgwm285-251bp、Xgwm165-166bp可显著提高小麦幼苗对2,4-滴丁酯、甲基二磺隆的抗性,典型载体品种分别为Niavt14和兰考298;Xcfd32-160bp/158bp、Xwmc617-157bp、Xmag1263-135bp和Xmag2137-189bp等位类型可显著提高小麦芽、根对甲基二磺隆的抗性,载体品种分别为兰考298、周麦16、济南17等;Xcfd76-208bp、Xcfd76-185bp和Xcfd49-131/143bp具有显著提高芽对2,4-滴丁酯的抗性,典型载体品种分别为农大876、新麦18及周麦16。
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| 关 键 词： | 小麦 除草剂抗性 分子标记 关联分析 |
| 收稿时间： | 2018/6/19 0:00:00 |
Exploring genetic loci and their elite alleles for herbicide resistance in common wheat by association analysis |
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| CHANG Cheng,LI Renhan,WU Qiong,ZHENG Biao,REN Fanfan,FANG Dongdong,ZHU Yulei,WANG Shengxing,LIU Kai,CHEN Xuejian and ZHANG Haiping.Exploring genetic loci and their elite alleles for herbicide resistance in common wheat by association analysis[J].Journal of Anhui Agricultural University,2019,46(1):104-110. |
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| Authors: | CHANG Cheng LI Renhan WU Qiong ZHENG Biao REN Fanfan FANG Dongdong ZHU Yulei WANG Shengxing LIU Kai CHEN Xuejian and ZHANG Haiping |
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| Institution: | School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036,School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036 and School of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture, Hefei 230036 |
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| Abstract: | |
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| Keywords: | Triticum aestivum L herbicide resistance molecular marker association analysis |
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