| 褐飞虱不同生物型基因组DNA的RAPD分析 |
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| 引用本文： | 许晓风,邹运鼎.褐飞虱不同生物型基因组DNA的RAPD分析[J].安徽农业大学学报,2000,27(1):5-8. |
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| 作者姓名： | 许晓风 邹运鼎 |
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| 作者单位： | 1. 南京农业大学植保系,南京,210095
2. 安徽农业大学研究生处 |
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| 基金项目： | 江苏省应用基础研究计划项目;BJ-97012; |
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| 摘 要： | 试验了两种ＤＮＡ提取方法抽提褐飞虱基因组ＤＮＡ的效果及其用所获得的ＤＮＡ进行ＲＡＰＤ扩增的技术条件，结果发现，采用蚜虫ＤＮＡ的提取方法（略有改进）来抽提高飞虱基因组ＤＮＡ，并用Ｓ系列的随机引物、ＲＡＰＤ的通用反应体系及９４℃变性，３６℃退火和７２℃延伸的扩增条件来对其进行随机扩增，可获得满意的效果，不同致害类群的群体间和同一致富类群的个体间的ＲＡＰＤ多态性均有差异，单雌纯化只能将个体间的差异缩小，
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| 关 键 词： | 褐飞虱 生物型 RAPD分析 基因组DNA 水稻害虫 |
| 文章编号： | 1000-2197(2000)01-0005-04 |
| 修稿时间： | 1999-09-24 |
Analysis on RAPD of Genome DNA in Different BPH Biotypes |
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| XU Xiao feng,CHENG Xia nian,ZU Yun ding.Analysis on RAPD of Genome DNA in Different BPH Biotypes[J].Journal of Anhui Agricultural University,2000,27(1):5-8. |
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| Authors: | XU Xiao feng CHENG Xia nian ZU Yun ding |
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| Institution: | XU Xiao feng 1,CHENG Xia nian 1,ZU Yun ding 2 |
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| Abstract: | A procedure of randomly amplified polymorphism DNA (RAPD) for analyzing genome DNA of brown planthopper (BPH), including the extraction method of DNA, amplifying reaction system and conditions etc was provided in this paper Moreover, the RAPD analysis on genome DNA of different BPH biotypes was conducted The results showed that there existed significant RAPD difference between various biotypes and among individual BPHs, and genetic purifying could lessen this difference in the level of individuals but not in the level of biotypes |
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| Keywords: | BPH biotype genome DNA RAPD analysis |
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