| 杂交榛子愈伤组织的诱导 |
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| 引用本文： | 汪青,丁之恩,常慧,许睿洁,周天驹,孙雨薇.杂交榛子愈伤组织的诱导[J].安徽农业大学学报,2017,44(6):1060. |
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| 作者姓名： | 汪青 丁之恩 常慧 许睿洁 周天驹 孙雨薇 |
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| 作者单位： | 安徽农业大学茶与食品科技学院，合肥 230036,安徽农业大学茶与食品科技学院，合肥 230036,安徽农业大学茶与食品科技学院，合肥 230036,安徽农业大学茶与食品科技学院，合肥 230036,安徽农业大学茶与食品科技学院，合肥 230036,安徽农业大学茶与食品科技学院，合肥 230036 |
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| 基金项目： | 安徽省林业厅世界银行五期项目“经济林混交林高效栽培及资源综合利用”资助。 |
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| 摘 要： | 利用组织培养技术对杂交榛子无性器官进行培养研究,从而为后续人工控制条件下获得更多的紫杉醇提供基础性研究材料。结果表明,对于10年生杂交榛子的根、茎段和叶片3种外植体进行愈伤组织诱导,其中茎段愈伤组织诱导率最高为66%,基础培养基MS为最适培养基,最适生长调节剂组合为2,4-D 2.5 mg·L-1+6-BA0.5 mg·L-1。
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| 关 键 词： | 杂交榛子 愈伤组织 诱导 诱导率 |
| 收稿时间： | 2016/8/18 0:00:00 |
Callus induction of cross-fibert |
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| WANG Qing,DING Zhien,CHANG Hui,XU Ruijie,ZHOU Tianju and SUN Yuwei.Callus induction of cross-fibert[J].Journal of Anhui Agricultural University,2017,44(6):1060. |
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| Authors: | WANG Qing DING Zhien CHANG Hui XU Ruijie ZHOU Tianju and SUN Yuwei |
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| Institution: | School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036,School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036,School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036,School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036,School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036 and School of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | In this study, vegetative tissues of cross-fibert were cultivated using tissue culture technology, which can provide a basic research for subsequently gaining more paclitaxel materials. The results indicated that in three explants (root, stem, and leaf) of a ten-year-old cross-fibert, the stem section had the highest callus induction rate, reaching to 66%. The best basal medium was MS and the optimal plant growth regulators were the combination of 2,4-D 2.5 mg·L-1+6-BA 0.5 mg·L-1. |
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| Keywords: | cross-fibert callus induction induction rate |
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