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| 家蚕对BmNPV抗病性与中肠消化酶活性的关系 | |
| 引用本文： | 刘震,鲍先巡,尤征英,杨莹,徐家萍.家蚕对BmNPV抗病性与中肠消化酶活性的关系[J].安徽农业大学学报,2009,36(3). |
| 作者姓名： | 刘震 鲍先巡 尤征英 杨莹 徐家萍 |
| 作者单位： | 1. 安徽农业大学生命科学学院,合肥230036;安徽省农业科学院,合肥230061 2. 安徽省农业科学院,合肥,230061 3. 安徽农业大学生命科学学院,合肥,230036 |
| 基金项目： | 安徽省科技攻关项目(08010302152);;安徽省国际合作项目(07080703023)共同资助 |
| 摘 要： | 对6个现行家蚕品种中肠消化酶活性与家蚕对BmNPV病毒抗性比较与分析,以期寻找现行家蚕品种消化酶的活性与抗性的关系,为辅助家蚕育种及品种推广奠定基础.以DNS法测定不同品种海藻糖酶活性,以Fo-lion-酚法测定不同品种蛋白酶活性.以及以铜皂法测定脂肪酶活性,同时对不同品种家蚕4龄起蚕经口添加BmN-PV病毒进行抗性调查分析.结果表明,蛋白酶、脂肪酶酶活顺序为664×663较低,667×668相对较高,其他品种处于中间层次,对应抗性测定结果的相关分析表明,活性越强抗性越强,呈显著正相关关系;而海藻糖酶的活性与抗性的关系没有较好的相关性.因此,家蚕中肠消化液的蛋白酶、脂肪酶可能与家蚕对BmNPV病毒抗性有关. |
| 关 键 词： | 家蚕 海藻糖酶 蛋白酶 脂肪酶 核型多角体病毒 抗病性 |
The relationship between the resistance of silkworm against BmNPV and the activity of digestive enzymes |
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| LIU Zhen,BAO Xian-xun,YOU Zheng-ying,YANG Ying,XU Jia-ping.The relationship between the resistance of silkworm against BmNPV and the activity of digestive enzymes[J].Journal of Anhui Agricultural University,2009,36(3). | |
| Authors: | LIU Zhen BAO Xian-xun YOU Zheng-ying YANG Ying XU Jia-ping |
| Institution: | 1.School of Life Science;Anhui Agricultural University;Hefei 230036;2.Anhui Academy of Agricultural Sciences;Hefei 230061 |
| Abstract: | In order to find out the relationship between digestive enzymes and the resistance of BmNPV and to help silkworm breeding,we compared and analyzed the relationship between the activity of digestive enzymes and the resistance of BmNPV about silkworm,using the method of DNS to detect the activity of trehalase,and Folion-phenol to detect the activity of protease,and using the method of copper soap to detect the activity of lipase.In order to survey the resistance of silkworm,BmNPV were added in artificial diet... |
| Keywords: | silkworm trehalase protease lipase BmNPV resistance |
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