| 喜树内生真菌的分离及其对植物病原菌的抑菌活性测定 |
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| 引用本文： | 蔡永欢,花日茂,柏钰,秦玲,操海群,吴祥为.喜树内生真菌的分离及其对植物病原菌的抑菌活性测定[J].安徽农业大学学报,2010,37(4). |
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| 作者姓名： | 蔡永欢 花日茂 柏钰 秦玲 操海群 吴祥为 |
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| 作者单位： | 安徽农业大学资源与环境学院,安徽省农产品安全重点实验室,合肥230036;安徽农业大学资源与环境学院,安徽省农产品安全重点实验室,合肥230036;安徽农业大学资源与环境学院,安徽省农产品安全重点实验室,合肥230036;安徽农业大学资源与环境学院,安徽省农产品安全重点实验室,合肥230036;安徽农业大学资源与环境学院,安徽省农产品安全重点实验室,合肥230036;安徽农业大学资源与环境学院,安徽省农产品安全重点实验室,合肥230036 |
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| 基金项目： | 安徽省学术技术带头人项目，安徽省科技攻关计划项目 |
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| 摘 要： | 采用组织分离法,以PDA培养基为分离培养基,从喜树的枝条、叶片、果实和根中分离获得28株内生真菌.对峙试验结果显示,28株内生真菌对6种植物病原菌均有不同程度的抑制作用;其中G-4菌株作用最明显,对苹果腐烂病菌(Valsa mali miyabe et Yamada)、茄褐纹病菌Phomopsis vexans(Sacc.et Syd.)Harter]的抑制率分别为89.68%和83.88%.对G-4菌株生物学特性测定结果表明,G-4菌株生长最适温度为28℃,最适pH范围为6.4～8;在麦芽浸粉培养基上G-4菌丝生长迅速;果糖为最佳碳源.
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| 关 键 词： | 喜树 内生真菌 抑菌活性 生物学特性 |
Isolation of endophytic fungus from Camptotheca acuminata Decne and its inhibition activities to plant pathogens |
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| CAI Yong-huan,HUA Ri-mao,BAI Yu,QIN Ling,CAO Hai-qun,WU Xiang-wei.Isolation of endophytic fungus from Camptotheca acuminata Decne and its inhibition activities to plant pathogens[J].Journal of Anhui Agricultural University,2010,37(4). |
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| Authors: | CAI Yong-huan HUA Ri-mao BAI Yu QIN Ling CAO Hai-qun WU Xiang-wei |
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| Institution: | CAI Yong-huan,HUA Ri-mao,BAI Yu,QIN Ling,CAO Hai-qun,WU Xiang-wei(School of Resources and Environment,Anhui Agricultural University,Key Laboratory of Agri-Food Safety of Anhui Province,Hefei 230036) |
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| Abstract: | Taken on potato dextrose agaras as medium,28 strains of endophytic fungi were isolated from the stems,leaves,fruits and roots of Camptotheca acuminata Decne with tissue isolation method.The results of plat-confront indicated that 28 strains of endophytic fungi had some inhibition on the six plant pathogens;the strain G-4 has significant effect on pathogens,and the inhibition rates to Valsa mali and Phomopsis vexans were more than 89.68% and 83.88%,respectively.Biological characteristics of strain G-4 was st... |
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| Keywords: | Camptotheca acuminata Decne endophytic fungi inhibition activities biological characteristics |
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