首页 | 本学科首页   官方微博 | 高级检索  
     检索      

不同分离方法获得的冬虫夏草无性型菌株的交配型 基因MAT1-2-1的初步分析
引用本文:张磊,肖岩岩,陈超,李春如.不同分离方法获得的冬虫夏草无性型菌株的交配型 基因MAT1-2-1的初步分析[J].安徽农业大学学报,2013,40(5):819.
作者姓名:张磊  肖岩岩  陈超  李春如
作者单位:安徽农业大学微生物防治省重点实验室,合肥 230036,安徽农业大学微生物防治省重点实验室,合肥 230036,安徽农业大学微生物防治省重点实验室,合肥 230036,安徽农业大学微生物防治省重点实验室,合肥 230036
基金项目:国家高技术研究发展计划(863计划)(2007AA021506)和教育部留学回国人员科研启动基金(2009年)共同资助。
摘    要:对采用不同分离方法获得的40个冬虫夏草(Ophiocordyceps sinensis)无性型菌株进行交配型基因MAT1-2-1的PCR特异扩增,并随机取其中7个拼接好的MAT1-2-1的碱基序列,与4个来自GenBank 数据在内的共11个样品构建了系统发育树。结果表明,采自青海的7个供试菌株,连同GenBank中来自青海的菌株(FJ654176)共同组成了一大类群,而GenBank中其他地区的菌株则聚成另外的类群;随机分离得到的10个单子囊孢子菌株全部含有MAT1-2-1基因, MAT1-1: MAT1-2未见按4:4进行分离,提示冬虫夏草极有可能是同宗配型。

关 键 词:冬虫夏草  无性型菌株  MAT1-2-1基因  单子囊孢子  同宗配型
收稿时间:3/4/2013 12:00:00 AM

Preliminary analysis of MAT1-2-1 mating type genes of anamorphic isolates from different sources of Ophiocordyceps sinensis
ZHANG Lei,XIAO Yan-yan,CHEN Chao and LI Chun-ru.Preliminary analysis of MAT1-2-1 mating type genes of anamorphic isolates from different sources of Ophiocordyceps sinensis[J].Journal of Anhui Agricultural University,2013,40(5):819.
Authors:ZHANG Lei  XIAO Yan-yan  CHEN Chao and LI Chun-ru
Institution:Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036,Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036,Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036 and Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036
Abstract:There is close relationship between the mating type gene of Ophiocordyceps sinensis and the artificial introduction of fruiting body. Many scholars focus on the mating type of O.sinensis, and MAT1-2-1 sequences of 40 isolates of O.sinensis by different methods were amplified with the primers MAT1-2-1 and evolutionary phylogenetic analyses were performed with sequences of the MAT1-2-1 gene, of which 7 sequences of MAT1-2-1 came from this experiment randomly and 4 sequences of MAT1-2-1 cited from the GenBank data. The result showed that 7 isolates collected from Qinghai, together with the isolates from Qinghai in GenBank make up a large group with the 96% bootstrap value and the isolates from the other area clustered into the other part. Meanwhile, all of single ascospore isolates of O.sinensis contain the MAT1-2-1 gene and the ratio of MAT1-1: MAT1-2 has not been found by 4:4. So O. sinensis might not be heterothallism, to a large extent, it is homothallic.
Keywords:Ophiocordyceps sinensis  anamorph  MAT1-2-1 gene  single ascospore  homothallic
点击此处可从《安徽农业大学学报》浏览原始摘要信息
点击此处可从《安徽农业大学学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号