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球孢白僵菌标准菌株的筛选及毒力效价的评定
引用本文:王成树,樊美珍,等.球孢白僵菌标准菌株的筛选及毒力效价的评定[J].安徽农业大学学报,1999,26(2):130-134.
作者姓名:王成树  樊美珍
作者单位:安徽农业大学森林利用学院
摘    要:观察、测定了来源不同的球孢白僵菌19个菌株的毒力、产孢量、萌发中时、水分活性,紫外照射活率、水浴活率等多个生物及生态学指标。从专业角度构建最优序列,经灰色分析,以供试菌株Bb54具最大灰关联度(r=0.9490),故将其确定为标准菌株。将标准菌株毒力与其它菌株相比较,得到不同菌株的毒力效价单位,其中以Bb13的效价最高,为1148.65,其次为Bb86,效价1125.16,两者的灰关联度分别为0.8813及0.8950。本文的研究结果为虫生真菌的标准化提供了方法上的参考。

关 键 词:球孢白僵菌  马尾松毛虫  毒力效价  微生物杀虫剂

Standard Strain Selection and Virulence Titer Assessment of Beauveria bassiana
Wang ChengshuFan MeizhenLi Zengzhi.Standard Strain Selection and Virulence Titer Assessment of Beauveria bassiana[J].Journal of Anhui Agricultural University,1999,26(2):130-134.
Authors:Wang ChengshuFan MeizhenLi Zengzhi
Abstract:Different biological and ecological characteristics,virulence,sporulation,half germination time (GT50),rapid dis and rehydration viabilities,UV radiation viabilities,bathing viabilities and colony growth rates of 19 strains of Beauveria bassiana were measured respectively.The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics.The Bb54 strain was determined as the type strain with highest grey relational indexes (r=09491).The different strain's virulence titer was calculated by comparing its virulence with the type strain's and the Bb13 strain showed the highest titer,e.g.114865,followed by Bb86 with 112516.Their grey relational indexes,08813 and 08950 respectively were acceptable.This study can make some contribution to settling down the criterion problems of entomopathogenic fungi preparation and formulation.
Keywords:Beauveria bassianaDendrolimus punctatusGrey relational    analysisStandard strainVirulence titer
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