黄山松成熟胚培养与植株再生技术研究

以黄山松(Pinus taiwanensis Hayata)成熟胚为试验材料，进行离体培养与植株再生条件的初步研究。结果表明，黄山松成熟胚外植体表面灭菌以75%的乙醇处理1 min，3%的次氯酸钠处理10 min为适宜；愈伤组织诱导培养基以MS + 1.0 mg·L^-1 6-BA + 0.2 mg·L^-1 NAA为最佳；适宜的不定芽分化培养基为DCR + 1.0 mg·L^-1 6-BA + 0.05 mg·L^-1 NAA；不定芽伸长以DCR + 0.1 mg·L^-1 6-BA + 0.05 mg·L^-1 NAA为适宜；生根培养基以1/2 DCR + 2.0 mg·L^-1 IBA + 0.05 mg·L^-1 NAA为适宜。建立的植株再生体系为黄山松种质资源的保存、遗传改良及优质种苗繁殖等研究提供了参考。

Research on mature embryo culture and plant regeneration of Pinus taiwanensis Hayata

In this study, the mature embryo of Pinus taiwanensis Hayata was used as the experimental material to conduct a preliminary study of in vitro culture and plant regeneration conditions. The results showed that the suitable surface sterilization method for mature embryos of P. taiwanensis Hayata was 75% alcohol treatment for 1 min and 3% sodium hypochlorite for 10 min; the optimal callus induction medium was MS + 1.0 mg·L^-1 6-BA + 0.2 mg·L^-1 NAA; the optimal induction medium for adventitious buds was DCR + 1.0 mg·L^-1 6-BA + 0.05 mg·L^-1 NAA; the suitable induction condition for adventitious bud elongation was DCR + 0.1 mg·L^-1 6-BA + 0.05 mg·L^-1 NAA; the optimal medium for rooting was 1/2 DCR + 2.0 mg·L^-1 IBA + 0.05 mg·L^-1 NAA. The results obtained in this study will be of great significance for the preservation, genetic improvement and high-quality seedling reproduction of P. taiwanensis Hayata germplasms.