| 小鼠Lrh-1基因CDS区序列克隆及分析 |
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| 引用本文： | 顾月琴,张伟,王利红.小鼠Lrh-1基因CDS区序列克隆及分析[J].安徽农业大学学报,2011,38(3):372-375. |
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| 作者姓名： | 顾月琴 张伟 王利红 |
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| 作者单位： | 江苏畜牧兽医职业技术学院; |
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| 基金项目： | 江苏省“333”工程培养资金项目（BRA2010162）, 博士基金（JQ2009002）和青年基金 (201006)共同资助。 |
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| 摘 要： | 肝受体类似物-1（liver receptor homolog-1,Lrh-1;NR5A2）是核受体的Ftz-F1亚家族成员,在胚胎发育、分化、胆固醇代谢、胆汁酸的动态平衡以及类固醇激素生成等都具有重要的作用。通过对健康的经产小鼠不同个体间Lrh-1基因CDS（Coding Sequence）特征域区测序及比对分析,结果发现,在LBD（Ligand binding domain）配体结合域区存在较大序列差异,有2种类型,1种与NM001159769相似,而另1种则与NG012313.1相似,两者序列差异较大,当翻译为多肽链后发现,第2种类型的序列中提前出现终止子,氨基酸数量相对减少83个,但这种缺失未导致该基因功能的丧失,表明该区段与Lrh-1蛋白质功能的不紧密性。
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| 关 键 词： | 肝受体类似物-1(Lrh-1) 小鼠 孤儿核受体 分子克隆 序列分析 |
| 收稿时间： | 2010/10/20 0:00:00 |
Cloning and sequence analysis of CDS of liver receptor homolog-1 from mice |
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| GU Yue-qin,ZHANG Wei and WANG Li-hong.Cloning and sequence analysis of CDS of liver receptor homolog-1 from mice[J].Journal of Anhui Agricultural University,2011,38(3):372-375. |
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| Authors: | GU Yue-qin ZHANG Wei and WANG Li-hong |
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| Institution: | GU Yue-qin,ZHANG Wei,WANG Li-hong(Jiangsu Animal Husbandry and Veterinary College,Taizhou225300) |
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| Abstract: | The liver receptor homolog-1(Lrh-1;NR5A2) is one of members of the Ftz-F1 subfamily of nu-clear receptors.Lrh-1 plays a predominant role in the embryo development,reverse cholesterol transport,bile-acid homeostasis and steroidogenesis.In this study,we analyzed the CDS of Lrh-1 from several mice by cloning and sequencing,and the result shows that there existed sequence differences in LBD region between different mice.There are two types in LBD region,one is similar to NM_001159769,and the other is similar to... |
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| Keywords: | liver receptor homolog-1 mouse orphan nuclear receptor molecular clone sequence analysis |
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