| 绵羊GDF9基因FecTT突变检测 |
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| 引用本文： | 耿彩霞,冯涛,储明星,方丽,狄冉,曹贵玲,黄冬维,唐倩倩,陈宏权.绵羊GDF9基因FecTT突变检测[J].安徽农业大学学报,2011,38(3):341-345. |
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| 作者姓名： | 耿彩霞 冯涛 储明星 方丽 狄冉 曹贵玲 黄冬维 唐倩倩 陈宏权 |
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| 作者单位： | 安徽农业大学生命科学学院;中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室; |
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| 基金项目： | 国家科技支撑计划(2008BADB2B01); 国家现代农业产业技术体系建设专项资金(农科教发[2008]10号); 国家重点基础研究发展计划(973)(2006CB102105); 中国农业科学院北京畜牧兽医研究所基本科研业务费专项(2010jc-9)和中国农业科学院首批优秀科技创新团队专项共同资助 |
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| 摘 要： | FecTT是冰岛Thoka绵羊GDF9基因外显子2编码区1279位发生的A→C突变(A1279C),导致编码GDF9蛋白C末端成熟肽第109位丝氨酸改变为精氨酸(S109R),该突变纯合子母羊不育,杂合子母羊产羔数比野生型多0.6只。本研究采用PCR-RFLP方法分析了GDF9基因FecTT突变在小尾寒羊、洼地绵羊、湖羊、考力代、白萨福克、黑萨福克、东弗里生、杜泊绵羊、特克塞尔、多赛特和中国美利奴11个绵羊品种中的分布。结果表明在11个绵羊品种中都没有检测到GDF9基因的FecTT突变,提示GDF9基因影响Thoka绵羊高繁殖力的FecTT突变对以上11个绵羊品种的繁殖力均没有显著影响。
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| 关 键 词： | 绵羊 GDF9基因 FecTT突变 PCR-RFLP |
Detection of the FecTT mutation of GDF9 gene in sheep |
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| GENG Cai-xi,FENG Tao,CHU Ming-xing,FANG Li,DI Ran,CAO Gui-ling,HUANG Dong-wei,TANG Qian-qian and CHEN Hong-quan.Detection of the FecTT mutation of GDF9 gene in sheep[J].Journal of Anhui Agricultural University,2011,38(3):341-345. |
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| Authors: | GENG Cai-xi FENG Tao CHU Ming-xing FANG Li DI Ran CAO Gui-ling HUANG Dong-wei TANG Qian-qian and CHEN Hong-quan |
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| Institution: | GENG Cai-xia1,FENG Tao2,CHU Ming-xing2,FANG Li2,DI Ran2,CAO Gui-ling2,HUANG Dong-wei2,TANG Qian-qian2,CHEN Hong-quan1(1.School of Life Sciences,Anhui Agricultural University,Hefei 230036,2.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193) |
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| Abstract: | FecTT mutation was a single base change(A1279C) in the coding region of exon 2 of GDF9(growth differentiation factor 9) gene in Icelandic Thoka sheep resulting in an amino acid change(S109R) in the C-terminus of the mature GDF9 protein.The ewes with heterozygous FecTT had 0.6 lambs per litter more than those with wild types.And ewes with homozygous FecTT were infertility in Thoka sheep.The aim of the present study was to detect the distribution of FecTT mutation of GDF9 gene in Small Tail Han,Wadi,Hu,Corrie... |
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| Keywords: | sheep growth differentiation factor 9 gene FecTT mutation PCR-RFLP |
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