| 克氏原螯虾酚氧化酶原mRNA组织分布特性研究 |
| |
| 引用本文： | 李艳和,王永杰,杨克礼,陈宇,佘磊.克氏原螯虾酚氧化酶原mRNA组织分布特性研究[J].安徽农业大学学报,2011,38(3):358-362. |
| |
| 作者姓名： | 李艳和 王永杰 杨克礼 陈宇 佘磊 |
| |
| 作者单位： | 安徽省农业科学院水产研究所;华中农业大学;动物胚胎工程及分子育种湖北省重点实验室; |
| |
| 基金项目： | 安徽省农科院院长青年创新基金资助 |
| |
| 摘 要： | 酚氧化酶原系统是甲壳动物体内与免疫相关的重要功能系统之一,对于甲壳动物的防御反应具有重要作用。本研究针对克氏原螯虾酚氧化酶原基因保守序列设计引物,利用实时荧光定量PCR法对克氏原螯虾酚氧化酶原mRNA的组织分布进行了初步研究。结果表明,克氏原螯虾酚氧化酶原基因在血淋巴、肝胰腺、卵巢和精巢中转录水平较高;在肌肉、表皮和鳃中有较弱表达;而在肠和胃中几乎无表达。此种组织表达特性可能与酚氧化酶原基因在机体生命活动过程中的功能有关。
|
| 关 键 词： | 克氏原螯虾 酚氧化酶原 mRNA 表达 |
Expression profile of proPO mRNA in red swamp crayfish, Procambarus clarkii |
| |
| LI Yan-he,WANG Yong-jie,YANG Ke-li,CHEN Yu and SHE Lei.Expression profile of proPO mRNA in red swamp crayfish, Procambarus clarkii[J].Journal of Anhui Agricultural University,2011,38(3):358-362. |
| |
| Authors: | LI Yan-he WANG Yong-jie YANG Ke-li CHEN Yu and SHE Lei |
| |
| Institution: | LI Yan-he1,2,WANG Yong-jie1,YANG Ke-li3,CHEN Yu1,SHE Lei1(1.Institute of Fishery,Anhui Academy of Agricultural Sciences,Hefei 230031,2.Huazhong Agricultural University,Wuhan 430070,3.Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Wuhan 430064) |
| |
| Abstract: | The prophenoloxidase system is considered to be an important mechanism of innate defence in crustacean.The expression profile of proPO mRNA in different tissues from P.clarkii was investigated by real time-PCR in this study.The results showed that the expression levels of proPO mRNA detected in haemocytes,hepatopancreas,ovary and spermary were higher than that in muscle,epidermis and branchia,while the proPO mRNA was almost not be observed in intestine and stomach.The characteristic of expression of the pro... |
| |
| Keywords: | Procambarus clarkii prophenoloxidase mRNA expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《安徽农业大学学报》浏览原始摘要信息 |
| 点击此处可从《安徽农业大学学报》下载免费的PDF全文 |
|
