瘤胃微生物体外降解不同来源肽的规律研究

通过体外试验分别研究36、72、144、216、324和450 mg·L-6个水平的大豆肽、玉米肽和瘤胃液肽在瘤胃微生物培养液中的降解规律.结果表明,随着培养时间的延长,3种肽的降解量均显著增加(P＜0.01或P＜0.05),而降解速率却显著降低(P＜0.01);当培养液中肽含量增加时,3种肽的降解量显著增加(P＜0.01或P＜0.05),降解速率显著提高(P＜0.01或P＜0.05),而降解率却显著下降(P＜0.05).对于不同肽源来说,培养初期瘤胃液肽的降解量以及降解速率显著高于玉米肽,培养后期几种来源肽的降解量以及降解速率差异不显著.因此,肽的来源和结构不同,其降解规律也有差异.

Degradation Patterns of Different Peptides in the Culture by Rumen Microorganisms

The degradation patterns of different peptides in the culture by rumen microorganisms were studied.The results showed that the amount of peptide degradation was increased significantly (P<0.01 or P<0.05) with the incubation time extended,but the rates of peptide degradation were decreased significantly (P<0.01). When peptide concentration was increased in the incubation , the amount of peptide degradation and rates of peptide degradation were increased significantly (P<0.01 or P<0.05),but the proportion of peptide degradation was decreased significantly (P<0.05).The amount,proportion and rates of rumen peptide degradation were higher than those of soybean protein peptide (P<0.05) and corn protein peptide (P<0.01).The results suggested that the rate of peptide degradation might be different with different proteins.