| siRNA对猪肝细胞THRSP基因mRNA表达的干扰效率 |
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| 引用本文： | 潘中婷,王学故,陈宏权,焦明慧,谢亚男,周梅,陈公伟,马帮军.siRNA对猪肝细胞THRSP基因mRNA表达的干扰效率[J].安徽农业大学学报,2014,41(3):363. |
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| 作者姓名： | 潘中婷 王学故 陈宏权 焦明慧 谢亚男 周梅 陈公伟 马帮军 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥230036;安徽农业大学动物科技学院,合肥230036;安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥230036 |
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| 基金项目： | 国家自然科学基金(31172180);安徽省科技攻关项目(1401032007,12010302052)共同资助 |
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| 摘 要： | 应用改良的剪切消化法(1%Ⅳ型胶原酶)消化分离仔猪肝脏细胞,建立稳定的仔猪肝脏细胞的分离与原代培养体系;通过siRNA途径及RT-PCR技术检测肝细胞THRSP基因mRNA表达水平。结果表明,平均每g肝脏可获得6.2×106个细胞,接种即时存活率达94.84%,培养存活率达50%左右,细胞转染后细胞存活率达80%以上;设计合成的siRNA的干扰效率为75.3%
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| 关 键 词： | 猪 肝脏细胞原代培养 siRNA THRSP基因 mRNA |
Interfered efficiency of siRNA on mRNA expression of THRSP gene in pig liver cells |
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| PAN Zhongting,WANG Xuegu,CHEN Hongquan,JIAO Minghui,XIE Yanan,ZHOU Mei,CHEN Gongwei and MA Bangjun.Interfered efficiency of siRNA on mRNA expression of THRSP gene in pig liver cells[J].Journal of Anhui Agricultural University,2014,41(3):363. |
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| Authors: | PAN Zhongting WANG Xuegu CHEN Hongquan JIAO Minghui XIE Yanan ZHOU Mei CHEN Gongwei and MA Bangjun |
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| Institution: | School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 and School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | |
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| Keywords: | pig liver cells in primary culture siRNA THRSP gene mRNA |
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