| 不同产脂能力猪脂肪合成相关基因表达谱分析 |
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| 引用本文： | 焦明慧,周梅,陈宏权,陈公伟,谢亚男,潘中婷,王学故,马帮军.不同产脂能力猪脂肪合成相关基因表达谱分析[J].安徽农业大学学报,2014,41(3):367. |
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| 作者姓名： | 焦明慧 周梅 陈宏权 陈公伟 谢亚男 潘中婷 王学故 马帮军 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥230036;安徽农业大学动物科技学院,合肥230036;安徽畜禽遗传资源保护与生物育种省级实验室,合肥230036 |
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| 基金项目： | 国家自然科学基金(31172180);安徽省科技攻关项目(1401032007,12010302052)共同资助 |
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| 摘 要： | 旨在筛查不同产脂能力猪脂肪合成的相关差异表达基因,分析其与猪THRSP基因表达的关联性。实验采集5头瘦肉型和3头脂肪型猪的肝脏,提取总RNA,利用基因表达谱芯片技术检测猪肝脏全基因组表达谱,分析THRSP基因与脂肪代谢相关差异表达基因之间的关联性。结果表明,相对瘦肉型猪,脂肪型猪脂肪酸合酶(FASN)、葡萄糖转运蛋白4(GLUT4)(P0.01)和乙酰辅酶A羧化酶α(ACACA)(P0.05)基因的表达显著上调,小GTP酶中Rab32和Rab4a显著上调,Rab27a则表现显著下调(P0.05);相关性分析显示GLUT4、FASN和ACACA基因表达的变化与THRSP基因呈显著正相关,Rab27a基因为显著负相关,Rab32和Rab4a基因则相关性不明显。
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| 关 键 词： | 基因表达谱 基因芯片 猪 脂肪沉积 THRSP基因 |
Gene expression analysis related to the fatty synthesis for the different fat deposition capacity in pigs |
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| JIAO Minghui,ZHOU Mei,CHEN Hongquan,CHEN Gongwei,XIE Yanan,PAN Zhongting,WANG Xuegu and MA Bangjun.Gene expression analysis related to the fatty synthesis for the different fat deposition capacity in pigs[J].Journal of Anhui Agricultural University,2014,41(3):367. |
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| Authors: | JIAO Minghui ZHOU Mei CHEN Hongquan CHEN Gongwei XIE Yanan PAN Zhongting WANG Xuegu and MA Bangjun |
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| Institution: | School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036; Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 and School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | This paper aims to screen the differentially expressed genes in fat synthesis associated with the fat deposition capacity in different pig-types, and to analyze their relationship with the expression of pig THRSP gene. The total RNA of the liver tissue samples from 5 lean- and 3 fat-type pigs were extracted, respectively. The differentially expressed genes were detected by using an Agilent microarray detection. The gene expression was related to THRSP gene. The results showed that comparing with lean-type pigs, the gene expressions of FASN, GLUT4 (P<0.01), and ACACA (P<0.05) in fat-type pigs were significantly up-regulated; and the gene expressions of Rab32 and Rab4a in small GTP enzyme were also significantly up-regulated, but Rab27a was significantly down-regulated (P<0.05). The expressions of the GLUT4, FASN and ACACA regulated genes related significantly to that of THRSP gene. |
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| Keywords: | gene expression profiling gene chip pigs fat deposition THRSP gene |
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